SN/T 4230-2015 English PDF

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SN/T 4230-2015: Quarantine protocol for haemophilus parasuis disease
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4230-2015	299	Add to Cart	3 days	Quarantine protocol for haemophilus parasuis disease	Valid

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Basic data

Standard ID: SN/T 4230-2015 (SN/T4230-2015)
Description (Translated English): Quarantine protocol for haemophilus parasuis disease
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 13,127
Date of Issue: 2015-05-26
Date of Implementation: 2016-01-01
Quoted Standard: GB/T 4789.28; GB/T 6682
Regulation (derived from): State Quality-Inspection-accreditation [2015] No.224
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the clinical diagnostic laboratory tests and Haemophilus parasuis disease. This standard applies to Haemophilus parasuis disease diagnosis and quarantine.

SN/T 4230-2015: Quarantine protocol for haemophilus parasuis disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Haemophilus parasuis disease quarantine technical specifications) People's Republic of China Entry-Exit Inspection and Quarantine Standards Haemophilus parasuis disease quarantine Technical Specifications Issued on. 2015-05-26 2016-01-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chongqing, People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Nantong University, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The main drafters of this standard. Choubao Feng, Wang Yu, Song Hongyan, Wang, the peak, the Secretary micro, Gubing Quan, high every knot, Liu Wenbin, Li Ying State. Haemophilus parasuis disease quarantine Technical Specifications

1 Scope

This standard specifies the clinical diagnostic laboratory tests and Haemophilus parasuis disease. This standard applies to Haemophilus parasuis disease diagnosis and quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.28 Microbiological examination of food staining, media and reagents Laboratory use specifications and test methods GB/T 6682 Analysis

3 medium and reagents

3.1 Water. in line with GB/T 6682 in a water specifications. 3.2 sterile defibrinated sheep blood. 3.3 sterile calf serum. 3.4 nicotinamide adenine dinucleotide (NAD). 3.5 tryptic soy agar (TSA). See A.1. 3.6 tryptic soy broth (TSB). See A.2. 3.7 chocolate agar plates. see A.3. 3.8 blood agar plates. see A.4. 3.9 Gram Staining Solution. See A.5. 3.10 biochemistry with media. According to the provisions of GB/T 4789.28 formulated. 3.11 Electrophoresis grade agarose powder. 3.12 TaqDNA polymerase. 5U/μL. 3.13 100bpDNAladderMarker (DNA molecular weight standard). 3.14 10-fold concentration TaqDNA polymerase buffer (10 × TaqBuffer). containing 15mmol/L magnesium chloride. 3.15 dNTP mixture. deoxyadenosine triphosphate (dATP), deoxythymidine triphosphate (dTTP), deoxyguanosine triphosphate (dGTP), off Oxygen triphosphate (dCTP) each 2.5mmol/L. 3.16 primer (HPS-F. 5'-GTGATGAGGAAGGGTGGTGT-3 '; HPS-R. 5'-GGCTTCGTCACCCTCTGT-3 '). 3.17 50 × TAE electrophoresis buffer. see A.6. 3.18 6 × loading buffer. see A.7. 3.19 ethidium bromide solution. see A.8. 3.20 Pig Haemophilus standard strain (type 1 to 15). supplied by the specified units. 3.21 Staphylococcus aureus (ATCC25923). 3.22 pleuropneumoniae Actinobacillus (ATCC27088). ......

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