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SN/T 4103-2015 English PDF

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SN/T 4103-2015: Method of polymerase chain reaction for detecting genetically modified pigs and their derived products
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4103-2015229 Add to Cart 3 days Method of polymerase chain reaction for detecting genetically modified pigs and their derived products Valid

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Basic data

Standard ID: SN/T 4103-2015 (SN/T4103-2015)
Description (Translated English): Method of polymerase chain reaction for detecting genetically modified pigs and their derived products
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X22
Classification of International Standard: 67.120.10
Word Count Estimation: 10,180
Date of Issue: 2015-02-09
Date of Implementation: 2015-09-01
Quoted Standard: GB/T 6682; GB/T 21101
Regulation (derived from): State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the transgenic pigs and processed GMO products Qualitative PCR detection method. This standard applies to transfer ��-3 fatty acid desaturase enzyme, phytase and knock the qualitative detection of myostatin gene and its processing products in pig genetically modified ingredients.

SN/T 4103-2015: Method of polymerase chain reaction for detecting genetically modified pigs and their derived products


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Qualitative PCR Detection and processed pig products, GMO) People's Republic of China Entry-Exit Inspection and Quarantine Standards Swine and processed products in qualitative GMO PCR detection method Issued on. 2015-02-09 2015-09-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, Beijing Institute of Animal Chinese Academy of Agricultural Sciences, the People's Republic of China State Shandong CIQ. The main drafters of this standard. Lin Xiangmei, Wang Qin, Wang Na, Liu, Zhu Zhen camp, Li Kui, Pandeng Ke, Sun Min, Fu Wei, Chousong Yin, Li Xiaolin. Swine and processed products in qualitative GMO PCR detection method

1 Scope

This standard specifies the transgenic pigs and processed GMO products Qualitative PCR detection method. This standard applies to transfer ω-3 fatty acid desaturase enzyme, phytase and knockout swine myostatin gene and its processed products transit Qualitative detection of gene components.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 21101 animal feed pigs derived materials PCR method 3 Terms, definitions and abbreviations The following abbreviations, terms and definitions apply to this document. 3.1 Terms and Definitions 3.1.1 Transgenic transgene This species does not have the will, from other species of functional DNA sequences, by introducing a variety of means to carry out the species Expression, in order for the species to obtain new varieties of features. 3.2 Acronyms β2M. β2-microglobulin (β2-microglobulin). MSTN. Myostatin (myostatin). Sfat-1. ω-3 fatty acid desaturase (omega-3fattyaciddesaturase). APPA. phytase (phytase). mtDNA. Swine DNA (mitochondrialDNA). TE. Tris · Cl, EDTA buffer. Tris. Tris (hydroxymethyl) aminomethane (tris (hydroxymethyl) aminomethane).

4 Reagents

4.1 PCR using primers Internal reference gene (pig mitochondrial DNA) primers were designed with reference to GB/T 21101, within the reference gene (pig β2M gene) exogenous gene primers
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