SN/T 4053-2014 English PDF

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SN/T 4053-2014: Quarantine protocol for chicken infectious anemia
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4053-2014	419	Add to Cart	4 days	Quarantine protocol for chicken infectious anemia	Valid

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Basic data

Standard ID: SN/T 4053-2014 (SN/T4053-2014)
Description (Translated English): Quarantine protocol for chicken infectious anemia
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 16,134
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB/T 6682
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the chicken infectious anemia (CIA) virus isolation and identification, polymerase chain reaction (PCR), fluorescence polymerase chain reaction (fluorescence PCR), indirect immunofluorescence assay (IFA) and enzyme-linked immunosor

SN/T 4053-2014: Quarantine protocol for chicken infectious anemia

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for chicken infectious anemia People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Chicken infectious anemia quarantine technical specification Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Supervision Committee. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Dongguan Entry and Exit Inspection of the People's Republic of China Epidemic situation. The main drafters of this standard. Sha Caihua, Yang Su, Chen Xuan, Xu Caizhen, Liang Yuying, Wang Wei, Chen Jinhui, Xu Haini, Luo Baozheng, Liao Xiuyun. Chicken infectious anemia quarantine technical specification

1 Scope

This standard specifies the isolation and identification of chicken infectious anemia (CIA) virus, polymerase chain reaction (PCR), fluorescent polymerase chain reaction Technical requirements for (fluorescence PCR), indirect immunofluorescence (IFA), and enzyme-linked immunosorbent assay (ELISA). This standard applies to the quarantine of chicken infectious anemia.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Virus isolation and identification

3.1 Reagents 3.1.1 Modified minimum element nutrient solution (DMEM) medium, see A.1 for preparation. 3.1.2 Marek's disease virus transformed lymphocyte line MDCC-MSB1 cells. 3.1.3 Standard positive serum. Serum obtained from SPF chickens immunized with CIAV antigen. 3.1.4 Standard negative serum. SPF chicken serum without CIAV infection. 3.1.5 Newborn bovine serum, penicillin, streptomycin. 3.2 Instruments and equipment 3.2.1 Carbon dioxide incubator. 3.2.2 Water bath. 3.2.3 Ordinary refrigerators and low temperature refrigerators. 3.2.4 Centrifuge. 3.2.5 Invert the microscope. 3.2.6 Micropipette, 2μL~20μL, 20μL~200μL. 3.2.7 Cell culture flask, pipette, centrifuge tube, grinding instrument, 0.2μm microporous membrane. 3.3 Sample collection and processing CIAV is present in tissues and organs such as liver, skin, spleen, thymus, bursa, kidney and bone marrow of sick chickens. Aseptically collecting the above tissue, 20% tissue suspension was prepared with serum-free DMEM, centrifuged at 3000r/min for 30min, and the supernatant was treated at 70°C for 5min. 10% chloroform was treated at room temperature for 15 min, centrifuged at 10,000 r/min for 20 min, and the supernatant was taken for CIAV virus isolation. ......

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