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SN/T 4043-2014 English PDF

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SN/T 4043-2014: PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4043-2014189 Add to Cart 3 days PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes Valid

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Basic data

Standard ID: SN/T 4043-2014 (SN/T4043-2014)
Description (Translated English): PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 7,742
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB 19489; WS 233; SN/T 1300; SN/T 1553; SN/T 1560; SN/T 1596; SN/T 1876
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the border crossings PCR detection method on mosquitoes carrying bancrofti and Brugia malayi, including sample testing procedures, test methods, and determines and reports. This standard applies to the inspection and quarantine ins

SN/T 4043-2014: PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Mosquitoes carry heartworm (bancrofti, Brugia malayi) of PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Mosquitoes carry heartworm (bancrofti, Brugia malayi) of PCR detection method Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China Exit Inspection and Quarantine and the State of Gansu, Sichuan, People's Republic of China Exit Inspection and Quarantine. Drafters of this standard. Tian Hong, Liu Lijuan, Liu Fang, high Yufeng, Chen Xiao Xiao, Zhang Hongbing. Mosquitoes carry heartworm (bancrofti, Brugia malayi) of PCR detection method

1 Scope

This standard specifies the border crossings PCR detection method on mosquitoes carrying bancrofti and Brugia malayi, including sample testing procedures to detect Method, and determines and reports. This standard applies to the inspection and quarantine institutions Mosquitoes carry bancrofti and Brugia malayi detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria SN/T 1300 frontier port monitoring procedures Mosquitoes SN/T 1553 into the exit of surveillance for aircraft medical vectors SN/T 1560 into the outward vessel monitoring procedures medical vectors SN/T 1596 exit into the vehicle monitoring procedures medical vectors SN/T 1876 medical vectors specimen collection, production and preservation regulations

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Filarial Filaria Spread by blood-sucking arthropods a class of parasitic nematodes. Adult parasites in vertebrate definitive host of the lymphatic system, subcutaneous tissue, abdominal Cavity, chest, etc.. In certain blood-sucking arthropods larvae intermediate host in vivo development. When these intermediate host vampire, mature infection Larvae that is, since its beak escape through the skin invade the final host body develop into adults. Filarial parasites in the human body there are eight kinds of popular Mainly Wuchereria bancrofti (Wuchereriabancrofti) and malayi (Brugiamalayi) based. Two similar patterns, are all parasites Milky silky, smooth surface, male and female variant. 3.2 Mosquitoes and Mosquito The main media bancrofti is Culex pipiens (Culexpipienspallens) and Culex quinquefasciatus (Cx.pipiensquinque- fasciatus), the main medium of Malay filariasis Anopheles sinensis (Anophelessinensis) and Anopheles Anopheles (An.anthropophagus) and the like. 3.3 PCR technology polymerasechainreaction PCR technique is a method of in vitro enzymatic synthesis of specific DNA fragments by denaturing high temperature, low temperature and optimum temperature annealing steps and other anti-extending It should form a cycle, cycle, so that the purpose of DNA can be amplified quickly, with high specificity, high sensitivity, easy to operate, saving time, etc.
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