SN/T 3993-2014 English PDF

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SN/T 3993-2014: Quarantine protocol for infection with batrachochytrium dendrobatidis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3993-2014	209	Add to Cart	3 days	Quarantine protocol for infection with batrachochytrium dendrobatidis	Valid

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Basic data

Standard ID: SN/T 3993-2014 (SN/T3993-2014)
Description (Translated English): Quarantine protocol for infection with batrachochytrium dendrobatidis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 8,869
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB/T 6682; GB/T 18088
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the clinical diagnosis of poison dart frog chytrid fungus infection, real time polymerase chain reaction detection method. This standard applies to poison dart frog chytrid fungus infection epidemiology, diagnosis, quarantine and m

SN/T 3993-2014: Quarantine protocol for infection with batrachochytrium dendrobatidis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Poison dart frog chytrid fungus infection quarantine technical specifications) People's Republic of China Entry-Exit Inspection and Quarantine Standards Poison dart frog chytrid fungus infection quarantine Technical Specifications Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is not equivalent part of the World Organisation for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2012 edition) chapter 2.1.1, etc. The relevant sections. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Xiamen Entry-Exit-Entry Inspection and Jilin People's Republic of China Quarantine Bureau. The main drafters of this standard. Xie star, Peng Xiaoli, Jian-Ping Shi, Liu Tong, Wang Qun force, Chen Shuangya, THE STAFF Xu Tyube, Sun Fuquan, Wang Jingming. Poison dart frog chytrid fungus infection quarantine Technical Specifications

1 Scope

This standard specifies the clinical diagnosis of poison dart frog chytrid fungus infection, real time polymerase chain reaction detection method. This standard applies to poison dart frog chytrid fungus infection epidemiology, diagnosis, quarantine and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling

3 Abbreviations

The following abbreviations apply to this document. bp. base pairs (basepair) DNA. deoxyribonucleic acid (deoxyribonucleicacid) dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate) FAM. fluorescent reporter group (Carboxyfluorescein) MGB. fluorescence quenching group (Minorgroovebinder) Real-timePCR. real time polymerase chain reaction (real-timepolymerasechainreaction) Taq. aquatic habitat heat bacteria (Thermusaquaticu)

4 instruments and reagents

4.1 Instrument 4.1.1 refrigerated centrifuge. 4.1.2 -20 ℃ cryogenic refrigerator. 4.1.3 tissue homogenizer. 4.1.4 real-time PCR reaction tube. 4.1.5 real-time PCR instrument. 4.1.6 autoclave. 4.1.7 UV spectrophotometer. 4.2 Reagents 4.2.1 Water should be consistent with GB/T 6682 in a water regulations. 4.2.2 DNA containing poison dart frog chytrid fungus or a plasmid containing the amplified region sequences. 4.2.3 TaqManFastUniversalPCRMasterMix (including dNTP, Taq polymerase and PCR reaction buffer, etc.). ......

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