SN/T 3987-2014 English PDF

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SN/T 3987-2014: Detection of specified risk materials of bovine spongiform encephalopathy(BSE)by real-time fluorescent RT-PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3987-2014	209	Add to Cart	3 days	Detection of specified risk materials of bovine spongiform encephalopathy(BSE)by real-time fluorescent RT-PCR method	Valid

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Basic data

Standard ID: SN/T 3987-2014 (SN/T3987-2014)
Description (Translated English): Detection of specified risk materials of bovine spongiform encephalopathy(BSE)by real-time fluorescent RT-PCR method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 8,882
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB/T 6682
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the special BSE risk material in real-time fluorescent RT-PCR detection method. This standard applies to qualitative detection of BSE in beef special risk material.

SN/T 3987-2014: Detection of specified risk materials of bovine spongiform encephalopathy(BSE)by real-time fluorescent RT-PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(BSE risk material special real-time fluorescent RT-PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards BSE risk material special real-time fluorescence RT-PCR detection method (BSE) byreal-timefluorescentRT-PCRmethod Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing Municipal Veterinary Drug Control, China National Conformity Assessment Accreditation Board. The main drafters of this standard. Shixi Ju, Li Bingling, Guo Zhihong, Ma Guiping, Su Zhiming, Liyan Xin, Liu Quanguo. BSE risk material special real-time fluorescence RT-PCR detection method

1 Scope

This standard specifies the special BSE risk material in real-time fluorescent RT-PCR detection method. This standard applies to qualitative detection of BSE in beef special risk material.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 Real-time fluorescence PCRrealtimefluorescentPCR Real time polymerase chain reaction. 3.1.2 Ct values cyclethreshold When the fluorescent signal of each reaction tube reaches a set threshold number of cycles experienced. 3.2 Acronyms The following abbreviations apply to this document. GFAP. glial fibrillary acidic protein (glialfibrilaryacidicprotein) FAM. 6- carboxyfluorescein (6-carboxyfluorescein) MGB. probe labeled with fluorescent group (minorgroovebinder) SRM. BSE specific risk material (specifiedriskmaterial)

4 main instruments

4.1 real-time PCR instrument. 4.2 high-speed desktop refrigerated centrifuge (centrifugal force up to 12000g above). 4.3 swirl oscillator. 4.4 refrigerator (2 ℃ ~ 8 ℃ refrigerator, -20 ℃ refrigerator, -80 ℃ refrigerator). 4.5 micropipette and various ancillary filter tips (10μL, 200μL, 1000μL). ......

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