SN/T 3964-2014 English PDF
Basic dataStandard ID: SN/T 3964-2014 (SN/T3964-2014)Description (Translated English): Detection and identification of Tomato severe leaf curl virus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.080 Word Count Estimation: 16,172 Date of Issue: 4/9/2014 Date of Implementation: 11/1/2014 Regulation (derived from): State inspection certification (2014) 210 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the method of quarantine and identification of immunology and molecular biology Tomato severe leaf curl virus. This standard applies to identify and quarantine may carry serious tomato leaf curl virus host plants and their products SN/T 3964-2014: Detection and identification of Tomato severe leaf curl virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Tomato severe leaf curl virus quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Standards Tomato severe leaf curl virus quarantine and identification methods Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Chinese Academy of Inspection and Quarantine, Beijing People's Republic of China Exit Inspection and Quarantine, the People's Republic of China State and Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China, People's Republic of China Yantai Entry-Exit Inspection Quarantine Bureau. The main drafters. Zhang Yongjiang, Deng Cong Liang, Shen Jianguo, Liao Furong, Su Zhi Ping, Lu Jie, water Zhu Fang, Li Mingfu, MATURAL Weimei Sheng. Tomato severe leaf curl virus quarantine and identification methods1 ScopeThis standard specifies the method of quarantine and identification of immunology and molecular biology Tomato severe leaf curl virus. This standard applies to identify and quarantine may carry serious tomato leaf curl virus host plants and their products.2 Tomato severe leaf curl virus basic informationName. Tomatosevereleafcurlvirus Abbreviation. ToSLCV Taxonomic position. The twin virus family (Geminiviridae), bean golden mosaic virus genus (Begomovirus). Transmission. mainly spread by whiteflies (Bemisiatabaci), it can also be spread by grafting. Tomato severe leaf curl virus For additional information, see Appendix A. Principle 3 According to specific reaction Tomato severe leaf curl virus and antibodies between samples were double-antibody sandwich enzyme-linked immunosorbent assay (DAS- ELISA) detection; conventional PCR or real-time PCR assay based on the specificity of the conserved regions of the viral DNA by electrophoretic bands large Little or amplification curve of the results of determination. 4 equipment, appliances, and reagents 4.1 Equipment Reader, ordinary PCR, real-time PCR instrument, multi-purpose electrophoresis, high-speed refrigerated centrifuge, gel imaging system, 4 ℃ ice Boxes, electronic analytical balance (0.001g), heated water bath, -80 ℃ ultra-low temperature refrigerators, autoclaves and ice machines. 4.2 major appliances Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL), centrifuge tubes, PCR tubes, microtiter plates and mortar and the like. 4.3 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. Immunosorbent assay enzyme-linked double antibody sandwich (DAS-ELISA) reagent Appendix B, conventional PCR detection reagent in Appendix C.5 quarantine and identification methods5.1 Preparation of the test sample 5.1.1 seedlings. symptoms (such as plant height, leaf malformation, curled, wrinkled, etc.) seedlings individually numbered, with each plant collection of symptoms blade 5g For subsequent detection; asymptomatic plants are divided into 10 per group and number of leaves at the top of 5g mixed collection for subsequent detection. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 3964-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 3964-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 3964-2014_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 3964-2014_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |