SN/T 3960-2014 English PDFUS$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3960-2014: Detection and identification of Blueberry stunt phytoplasma Status: Valid
Basic dataStandard ID: SN/T 3960-2014 (SN/T3960-2014)Description (Translated English): Detection and identification of Blueberry stunt phytoplasma Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.080 Word Count Estimation: 12,130 Date of Issue: 4/9/2014 Date of Implementation: 11/1/2014 Regulation (derived from): State inspection certification (2014) 210 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the dwarf blueberry quarantine and identification of phytoplasma. This standard applies to entry and exit quarantine and identification of tree seedlings blueberries and blueberry products blueberry dwarf phytoplasma. SN/T 3960-2014: Detection and identification of Blueberry stunt phytoplasma---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Blueberry dwarf phytoplasma quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Standards Blueberry dwarf phytoplasma quarantine and identification methods Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Li Xin, Lin Changjun, Wangyou Fu, a ginger, Cao Dongmei, Hu Qiang, Liu Hui Qiu, Wang Xiufen, Xufeng Min, Mu Haiqing. Blueberry dwarf phytoplasma quarantine and identification methods1 ScopeThis standard specifies the dwarf blueberry quarantine and identification of phytoplasma. This standard applies to entry and exit quarantine and identification of tree seedlings blueberries and blueberry products blueberry dwarf phytoplasma.2 blueberry dwarf phytoplasma basic informationEnglish name. Blueberrystuntphytoplasma Taxonomic position. Bacteria soft wall door (Tenericutes), flexible membrane Proteobacteria (Molicutes), non-steroidal MLO mesh (Acholeplasmatales), Nonsteroidal Mycoplasma Branch (Acholeplasmataceae), phytoplasma genus (Phytoplasma), 16SrⅠ phytoplasma group D subgroups. For additional information, see Appendix blueberry dwarf phytoplasma A.3 PRINCIPLE OF THE METHODBlueberry dwarf phytoplasma biological characteristics and genetic characteristics of the standard identification methods as the main basis. Morphological DAPI staining after preliminary screening methods; to phytoplasma ribosomal 16SrDNA universal primers for PCR amplification, Sequencing and restriction endonuclease fingerprinting as the main basis for judgment.4 instruments and appliances Reagents4.1 Instrument PCR instrument, clean benches, autoclaves, ice machines, refrigerated centrifuge desktop, small desktop centrifuge, ultra-low temperature freezer, conventional ice Box, a vortex, micro-injector, electrophoresis, gel imaging system, fluorescence microscopy, transmission electron microscopy, thin slicing machines. 4.2 Appliances Pipettes, mortar, centrifuge tubes, PCR tubes, graduated cylinders, beakers alcohol lamp, tweezers. 4.3 Reagents 4.3.1 CTAB buffer CTAB 20g/L Tris-HCl 100mmol/L (pH8.0) EDTA 20mmol/L (pH8.0) NaCl 1.4mol/L PVP-40 10g/L 4.3.2 PBS buffer (0.2mol/LpH7.4) Anhydrous NaH2PO4 24g ...... |
