SN/T 3963-2014 English PDF
Basic dataStandard ID: SN/T 3963-2014 (SN/T3963-2014)Description (Translated English): Detection of Peach rosette mosaic virus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.080 Word Count Estimation: 16,113 Date of Issue: 4/9/2014 Date of Implementation: 11/1/2014 Regulation (derived from): State inspection certification (2014) 210 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the method for inspection and identification peach rosette mosaic virus. This standard applies to quarantine and identification of peach, grapes and other plant material peach rosette mosaic virus. SN/T 3963-2014: Detection of Peach rosette mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Peach rosette mosaic virus detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Peach rosette mosaic virus detection methods Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Zhejiang Exit Inspection and Quarantine, the People's Republic of China State and Tianjin Entry-Exit Inspection and Quarantine, Ningbo People's Republic of China. The main drafters of this standard. ETHNIC SOCIETY OF Guo Jingze, Wen Weigang, Liming Fu, Zhu Shuifang. Peach rosette mosaic virus detection methods1 ScopeThis standard specifies the method for inspection and identification peach rosette mosaic virus. This standard applies to quarantine and identification of peach, grapes and other plant material peach rosette mosaic virus. Principle 2 Peach rosette mosaic virus Scientific name. Peachrosettemosaicvirus, Abbreviation. PRMV. Its specific groups of the virus coat protein Specificity of the genomic nucleic acid sequence is the main basis of this standard-setting. Related Information See Appendix A. 3 equipment, appliances, and reagents 3.1 Equipment ELISA detector, real-time PCR instrument, low temperature refrigerators, incubators, autoclaves, electronic analytical balance, high-speed desktop centrifuged Machine, constant temperature water bath, ice-making machines. 3.2 Appliances Micro adjustable pipette (range, respectively 2.5μL, 10μL, 100μL, 200μL and 1mL) and sterilized tip, 1.5mL centrifugation Tube, mortar, ELISA plate, 0.2mLPCR tubes, 96-well PCR reaction plates and lids. 3.3 Reagents Reagents, buffers, primers and probe sequences, etc. See Appendix B and Appendix C.4 Detection and Identification4.1 Serology (double antibody sandwich enzyme-linked immune assay) 4.1.1 Coating antibody The PRMV antibody with the coating buffer was diluted to working concentration, enzyme-linked plate, each hole by adding 100μL. The ELISA plate stamped (Or sealed with plastic wrap) into the humidor, placed in 37 ℃ incubator incubated for 2h (or 4 ℃ refrigerator overnight). After each well was added 300μLPBST washed three times, each time 3min. 4.1.2 Sample Preparation Plant leaves to be tested was weighed 0.2g, was added 2mL sample extraction buffer, followed by sufficient grinding, into a 1.5mL centrifuge tube, 5000r/min centrifugal 5min, the supernatant 1.10 dilution of the sample is a crude extract. 4.1.3 sample The sample was added to the crude extract of enzyme-linked antibody-coated plates, each well 100μL. Konga buffer as control, at the same time set ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 3963-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 3963-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). 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