SN/T 3962-2014 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3962-2014	479	Add to Cart	3 days	Detection and identification of Narcissus late season yellows virus	Obsolete

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Basic data

Standard ID: SN/T 3962-2014 (SN/T3962-2014)
Description (Translated English): Detection and identification of Narcissus late season yellows virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 12,185
Date of Issue: 4/9/2014
Date of Implementation: 11/1/2014
Regulation (derived from): State inspection certification (2014) 210
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the yellows virus testing procedures and methods Narcissus late season. This standard applies to inbound and outbound detection narcissus Narcissus late season yellows virus.

SN/T 3962-2014: Detection and identification of Narcissus late season yellows virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Quarantine and identification of late season yellow daffodils virus) People's Republic of China Entry-Exit Inspection and Quarantine Standards Quarantine and identification of Narcissus late season yellows virus Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine, Fujian Agriculture and Forestry University. The main drafters of this standard. Shen Jianguo, Liao Furong, Mark dispute, Cai Wei, Li Min, Zhang lights, Yan Cheng, Zhang Yongjiang, Gao Fang Luan, Zu Jian. Quarantine and identification of Narcissus late season yellows virus

1 Scope

This standard specifies the yellows virus testing procedures and methods Narcissus late season. This standard applies to inbound and outbound detection narcissus Narcissus late season yellows virus. Principle 2 Serological and molecular biological characteristics Narcissus late season yellows virus is the main basis of the development of methods for identification of quarantine. Narcissus late season yellows Taxonomic status of the virus, host range, disease symptoms, geographical distribution, transmission, see Appendix particle morphology, genomic and other A. 3 equipment, appliances, and reagents 3.1 Equipment High-speed refrigerated centrifuge, electronic balance (1/1000g), PCR instrument, electrophoresis, horizontal electrophoresis tank, -20 ℃ and -80 ℃ low temperature freezer Ultra-low temperature freezer, gel imaging analysis system, pH meter, a microwave, a magnetic stirrer, constant temperature water bath, autoclaves, clean benches, enzymes Standard instrument. 3.2 Appliances Various measurement ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL), PCR reaction tube, RNase-free from Heart tube (1.5mL), mortar, ELISA plates. 3.3 Reagents ELISA plate antigen capture enzyme-linked immunosorbent assay (plate-trappedantigenELISA, PTA-ELISA) reagent in Appendix B, Nested RT-PCR reagents in Appendix C.

4 Detection and Identification

4.1 PTA-ELISA Specific methods in Appendix B. 4.2 nested RT-PCR Specific methods, see Appendix C.

5 results found recording

5.1 Result judgment When yellows virus PTA-ELISA test results were positive Narcissus late season, and nested RT-PCR test results are positive, then the sentence ......

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