SN/T 3926-2014 English PDF

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SN/T 3926-2014: Determination of protein content in dairy foods, egg foods and soy foods for export - Coomassie brilliant blue method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3926-2014	209	Add to Cart	3 days	Determination of protein content in dairy foods, egg foods and soy foods for export - Coomassie brilliant blue method	Valid

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GB 5009.271   GB 5009.22   SN/T 2641   SN/T 3928   SN/T 3929   SN/T 3925   

Basic data

Standard ID: SN/T 3926-2014 (SN/T3926-2014)
Description (Translated English): Determination of protein content in dairy foods, egg foods and soy foods for export - Coomassie brilliant blue method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 8,895
Date of Issue: 4/9/2014
Date of Implementation: 11/1/2014
Regulation (derived from): State inspection certification (2014) 210
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method for determination of export Coomassie Brilliant Blue milk, eggs, beans in protein content. This standard applies to milk, eggs, beans determination of protein content.

SN/T 3926-2014: Determination of protein content in dairy foods, egg foods and soy foods for export - Coomassie brilliant blue method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Exports of milk, eggs, beans in the determination of protein content Coomassie brilliant blue) People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of export milk, eggs, legumes Protein Content Coomassie brilliant blue Determinationofproteincontentindairyfoods, eggfoodsandsoyfoodsforexport- Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhenjiang Exit Inspection and Quarantine. The main drafters of this standard. Circumstances of Li Ping, Zhou Hongbin, even Zhu Jin, Wu Xiaohua, Wang Zuxiang, Sun Li, Yu Yang, Shen Bo, Wanrong, Wang Hui. Determination of export milk, eggs, legumes Protein Content Coomassie brilliant blue

1 Scope

This standard specifies the method for determination of export Coomassie Brilliant Blue milk, eggs, beans in protein content. This standard applies to milk, eggs, beans determination of protein content.

2 Method summary

Coomassie brilliant blue G-250 after binding with proteins in dilute acid solution turns blue, the maximum absorption wavelength from 465nm into 595nm, Its blue dye protein complexes and protein content absorbance at 595nm wavelength is proportional.

3 Reagents and materials

Unless otherwise specified, reagents were analytical pure, distilled water. 3.1 Ethanol (95%). 3.2 phosphoric acid (98%). 3.3 Coomassie brilliant blue G-250. 3.4 Coomassie brilliant blue G-250 solution. Weigh about 100mg Coomassie Brilliant Blue G-250, dissolved 50mL95% ethanol, then add 100mL85% phosphoric acid diluted with water to 1L, filter paper. 3.5 Standards. Bovine serum albumin (BSA), a purity of 99.0% or greater. 3.6 bovine serum albumin (BSA) standard solution. Weigh bovine serum albumin 50mg, dissolved in water and dilute to 500mL, preparation Protein standard solution into 0.1mg/mL of.

4 instruments and equipment

4.1 UV-Vis spectrophotometer. Wavelength range. 190nm ~ 900nm. 4.2 Balance. a sense of the amount of 0.0001g and 0.001g. 4.3 ultrasonic cleaner. 4.4 centrifuge. speed of not less than 4000r/min. 4.5 plug tube. 10mL. 4.6 food grinder.

5 measuring step

5.1 Sample Preparation 5.1.1 liquid sample Weigh and mix the sample 1g (accurate to 0.001g) in 100mL flask with water to volume. A portion of the solution was 4000r/min from ......

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