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SN/T 3872-2014 English PDF

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SN/T 3872-2014: Detection of four pathogens in food for export. MALDI-TOF-MS method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 3872-2014209 Add to Cart 3 days Detection of four pathogens in food for export. MALDI-TOF-MS method Valid

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GB 4789.10   GB 4789.1   SN/T 1888.4   SN/T 5640   SN/T 5647   SN/T 5594   

Basic data

Standard ID: SN/T 3872-2014 (SN/T3872-2014)
Description (Translated English): Detection of four pathogens in food for export. MALDI-TOF-MS method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 07.100.30
Word Count Estimation: 8,852
Quoted Standard: GB 4789.4; GB 4789.7; GB 4789.30; GB/T 6682; GB 19489; SN 0170; SN/T 0173; SN/T 0184.1; SN/T 1022; NMKL NO.156
Regulation (derived from): AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine
Issuing agency(ies): General Administration of Customs
Summary: This Standard specifies the MALDI-TOF-MS detection of four pathogens in food. This Standard applies to foods Salmonella, Listeria monocytogenes bacterium, Vibrio parahaemolyticus, Vibrio cholerae four rapid detection of pathogens, other pathogens may refe

SN/T 3872-2014: Detection of four pathogens in food for export. MALDI-TOF-MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of four pathogens in food for export. MALDI-TOF-MS method People's Republic of China Entry-Exit Inspection and Quarantine Standards Export food pathogen detection method of Four MALDI-TOF-MS method Issued on. 2014-01-13 2014-08-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Juan Cao occasion, Cheng Chiu, Dong Weifeng, Cao Dongmei, Huang Daliang, Zhao Xin, Ma Lidan. Export food pathogen detection method of Four MALDI-TOF-MS method

1 Scope

This standard specifies the MALDI-TOF-MS detection of four pathogenic bacteria in food. This standard applies to food Salmonella, Listeria monocytogenes bacteria, Vibrio parahaemolyticus, Vibrio cholerae four kinds of pathogens Rapid detection of other pathogens can refer to use.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. National Food Safety Standard GB 4789.4 Microbiological Examination of Salmonella food GB 4789.7 national food safety standards of food microbiology testing Vibrio parahaemolyticus test National Food Safety Standard GB 4789.30 Microbiological examination of food monocytogenes Listeria test Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety SN 0170 export food Salmonella (bacteria including Arizona) test method SN/T 0173 Importers and Vibrio parahaemolyticus in food testing methods SN/T 0184.1 import and export food monocytogenes Listeria detection methods SN/T 1022 Vibrio cholerae in food import and export inspection methods NMKLNo.156 food in the detection and enumeration of Vibrio

3 Abbreviations

The following abbreviations apply to this document. 3.1 MALDI-TOF-MS. Matrix-AssistedLaserDesorption/IonizationTime-of-FlightMassSpectrome- try, matrix-assisted laser desorption ionization time of flight mass spectrometry. 3.2 CHCA. α-cyano-4-hydroxy-cinnamicacid, α- cyano-4-hydroxy cinnamic acid. 3.3 ACN. acetonitrile, acetonitrile. 3.4 TFA. trifluoroaceticacid, trifluoroacetic acid.

4 Method summary

MALDI-TOF-MS is applied to the rapid detection of bacterial whole cell technology, based primarily on features present in the bacterial surface proteins Molecular weight information, the completion of the identification and classification of bacteria. Dot matrix solution will be identified colonies sample with a suitable amount of added sample plate, the solvent evaporated After the formation of hair sample matrix co-crystallized by laser radiation as an energy source co-crystals, matrix molecules absorb energy and sample desorption
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