SN/T 3739-2013 English PDFUS$319.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3739-2013: Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR Status: Valid
Basic dataStandard ID: SN/T 3739-2013 (SN/T3739-2013)Description (Translated English): Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: C62 Classification of International Standard: 11.020 Word Count Estimation: 12,12 Quoted Standard: List of human infectious pathogens Adopted Standard: List of human infectious pathogens Regulation (derived from): National quality recognition (2013) 569 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the border exit port personnel or tick-borne encephalitis virus carrying real-time fluorescent RT-PCR detection, including detection of objects, specimen collection and handling, testing procedures, the results of the determination SN/T 3739-2013: Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR People's Republic of China Entry-Exit Inspection and Quarantine Standards Border crossings tick-borne encephalitis virus detection methods Real-time fluorescent RT-PCR method Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine of Jilin People's Republic of China Bureau of Shanghai Jiang Biological Technology Co., Ltd.. The main drafters of this standard. Li Zhihui, Zhan Xi Jing, Daniel Yeung, Yang Shunyi, sinus Yong Ying, Liu Yang, Zhuxu Ping, Zhu Qin Wei. Border crossings tick-borne encephalitis virus detection methods Real-time fluorescent RT-PCR method1 ScopeThis standard specifies the border ports of entry and exit personnel or tick-borne encephalitis virus carrying real-time fluorescent RT-PCR detection, including the detection of Like, specimen collection and handling, testing procedures, the results of determination. This standard applies to the border port of entry and exit personnel or tick-borne encephalitis virus carrying laboratory tests.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Human infection of pathogenic microorganisms directory (Department of Health 2006)3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 Tick-borne encephalitis virus tick-borneencephalitisvirus Russian spring-summer encephalitis virus Russianspring-summerencephalitisvirus Flaviviridae Flavivirus member, single-strand RNA viruses, mRNA full length 10886bp. The virus occurs According to school system Europe is divided into subtypes, the Far East and Siberia subtype subtypes three subtypes. The virus caused encephalitis is a natural focal disease. The virus reservoir host of many, the media mainly ticks, usually caused by susceptible people carrying the virus after being bitten by a tick disease. 3.2 Real-time fluorescent RT-PCR real-timeRT-PCR There are a variety of real-time fluorescent RT-PCR method, this standard uses a TaqMan hydrolysis probe method, the principle is the conventional RT-PCR On the basis of adding a specific fluorescent probe which is an oligonucleotide, the two sides were marked with a fluorescent reporter group and a A fluorescence quenching group. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quencher, PCR amplification using Taq enzyme 5'-3 'exonuclease activity of the enzyme hydrolysis probes, the fluorescent reporter group and fluorescence quenching group separation, the fluorescence monitoring system can receive The fluorescence signal. Each through a PCR cycle, the fluorescence signal is also the same fragment, there is a coincident index increased process signals Nucleic acid content is proportional to the intensity of the detected object, it can calculate the amount of detected objects according to a mathematical model.4 AbbreviationsThe following abbreviations apply to this document. BHQ. black hole quencher (blackholequencher) Ct values. cycle threshold values, each reaction tube fluorescent signal cycle number (cyclethreshold) set threshold experienced DEPC. diethyl pyrocarbonate (diethylpyrocarbonate) FAM. 6- carboxy-fluorescein, a fluorescent reporter group (6-carboxy-fluorescein) ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 3739-2013_English be delivered?Answer: Upon your order, we will start to translate SN/T 3739-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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