SN/T 2678-2010 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2678-2010	359	Add to Cart	3 days	Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay	Obsolete

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Basic data

Standard ID: SN/T 2678-2010 (SN/T2678-2010)
Description (Translated English): Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X20
Classification of International Standard: 67.120.30
Word Count Estimation: 9,919
Date of Issue: 2010-11-01
Date of Implementation: 2011-05-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Quality Inspection (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of fish, shrimp, shellfish and other fresh products microcystin enzyme-linked immunosorbent assay method. This standard applies to export fish, shrimp, shellfish and other freshwater products in the detection of microcystins.

SN/T 2678-2010: Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of microcystin in fresh water products for import and export.Enzyme-linked immunosorbent assay People's Republic of China Entry-Exit Inspection and Quarantine Standards Importers and freshwater products Microcystins Detection ELISA Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine Qingdao, People's Republic of China Bureau, Chinese Academy of Sciences Institute of Hydrobiology, People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Wu Zhenxing, only Li Wei, Lu Qing, Gannan Qin, Song Lirong, Chen Wei, Cao occasion Juan, Wangqiu Yan. Importers and freshwater products Microcystins Detection ELISA

1 Scope

This standard specifies the ELISA detection method and export fish, shrimp, shellfish and other freshwater products of microcystin. This standard applies to the import and export of fish, shrimp, shellfish and other freshwater products in the detection of microcystin.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Method summary

This standard aqueous methanol to extract freshwater products microcystin, after solid phase extraction (SPE) process, sample microcapsule Algal toxin microcystin enzyme markers common competitive binding antibody previously coated on the microplate, repeated washing liquid to clean all pores, and then After addition of chromogenic substrate, in order to then determined by comparing the absorbance values of the absorbance of the sample is worth microcystin content.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.1 microcystin detection kit (see Appendix A). 4.2 methanol. 4.3 Sodium hydroxide (NaOH). 4.4 Disodium hydrogen phosphate (Na2HPO3 · 12H2O). 4.5 extract. 70% aqueous methanol. 4.6 Eluent. 20% methanol aqueous solution. 4.7 Sodium hydroxide solution. Weigh sodium hydroxide (4.3) 0.4g, dissolved in water and dilute to 100mL. 4.8 phosphate buffer solution. Weigh disodium hydrogen phosphate (Na2HPO3 · 12H2O) (4.4) 34g, dissolved in water and dilute to 1L, hydroxide Sodium hydroxide solution (4.7) adjusted to pH 8.0. 4.9 microcystin standards. purity greater than or equal to 95%. 4.10 microcystin standard stock solution. methanol as solvent formulated as a stock solution of 100μg/L, and stored at -20 ℃ condition. 4.11 SPE. SEP-PAKCLASSICC18 (or equivalent). 5. Apparatus 5.1 Balance. a sense of the amount of 0.01g. ......

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