SN/T 2617-2022 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2617-2022	RFQ	ASK	3 days	(Quarantine and Identification Method of Winter Phytophthora)	Valid
SN/T 2617-2010	479	Add to Cart	3 days	Detection and identification of Phytophthora hibernalis Carne	Obsolete

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Basic data

Standard ID: SN/T 2617-2022 (SN/T2617-2022)
Description (Translated English): (Quarantine and Identification Method of Winter Phytophthora)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.20
Date of Issue: 2022-07-07
Date of Implementation: 2023-02-01
Issuing agency(ies): General Administration of Customs

SN/T 2617-2010: Detection and identification of Phytophthora hibernalis Carne

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora hibernalis Carne People's Republic of China Entry-Exit Inspection and Quarantine Standards Winter Health quarantine and identification of Phytophthora Issued on. 2010-05-27 2010-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Chinese Academy of Inspection and Quarantine. Drafters of this standard. Wu Shan goods, Du Hongzhong, Yan Jin. Winter Health quarantine and identification of Phytophthora

1 Scope

This standard specifies the phytosanitary born in winter Phytophthora PhytophthorahibernalisCarne quarantine authentication methods. This standard applies to pathogen detection for Winter Health Phytophthora host seedlings, branches, leaves, fruits and entrained soil winter green Phytophthora Phytophthora identification. Principle 2 According to Winter Health Phytophthora symptoms on the host, morphological characteristics and PCR amplified fragment was identified specific basis. The bacteria which He related information, see Appendix A.

3 Instrument

3.1 biological microscope, stereo microscope. 3.2 Light biological incubator. 3.3 autoclave. 3.4 high-speed centrifuges. 3.5 PCR amplification, electrophoresis, gel imager. 4 reagents, materials and media 4.1 Reagents 4.1.1 horses vancomycin, rifampin sodium, benomyl, hymexazol, calcium carbonate (CaCO3), β- sitosterol, tryptophan, sulfur, ammonia, calcium chloride (CaCl2 · H2O), sodium hypochlorite (NaClO). 4.1.2 Agarose, of SDS (sodium dodecyl sulfate), Tris-HCl, magnesium chloride, hydrochloric acid, EDTA (ethylenediaminetetraacetic acid tetrasodium salt), hydroxide Sodium acetate, sodium chloride, Tris, CTAB (cetyl trimethyl ammonium bromide), TAE, ethanol, phenol, chloroform, isopropanol, Iso-amyl alcohol, SYBRGreenⅠ nucleic acid dyes. 4.1.3 proteinase K, TaqDNApolymerase, dNTP, DNA molecular weight Marker. 4.1.4 PCR primers PHIB1/PHIB2. 4.2 Material Agar, V8 liquid, cedar (Cedrusdeodara) pine needle, citrus (Citrusspp.) Blade, carrot, corn oil. 4.3 Medium The standard medium used, see Appendix B. ......

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