SN/T 2497.9-2010 English PDF

US$139.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 2497.9-2010: Test method of import and export dangerous chemicals. Part 9: Determination of serum hemolysin
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2497.9-2010	139	Add to Cart	2 days	Test method of import and export dangerous chemicals. Part 9: Determination of serum hemolysin	Valid

Similar standards

GB/T 35925   GB/T 4472   GB/T 3143   SN/T 2497.3   SN/T 2497.11   SN/T 2497.10   

Basic data

Standard ID: SN/T 2497.9-2010 (SN/T2497.9-2010)
Description (Translated English): Test method of import and export dangerous chemicals. Part 9: Determination of serum hemolysin
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: G09
Classification of International Standard: 13.300
Word Count Estimation: 5,544
Date of Issue: 2010-03-02
Date of Implementation: 2010-09-16
Quoted Standard: GB 14924.1; GB 14925
Regulation (derived from): National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the determination of serum hemolysin test terminology and definitions, test theory, test methods and test results. This standard applies to hemolysin assay detects import of dangerous chemicals in mice to sheep red blood cell hemolysin impact.

SN/T 2497.9-2010: Test method of import and export dangerous chemicals. Part 9: Determination of serum hemolysin

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of import and export dangerous chemicals.Part 9. Determination of serum hemolysin Exit inspection and quarantine industry standard book People's Republic of China Import and export of dangerous chemicals - Test methods Part 9. Determination of serum hemolysin test Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts. --- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test; --- Part 2. plaque-forming cells (PFC) test; --- Part 3. Large Zhuan reproduction test; --- Part 4. Saccharomyces cerevisiae mitotic recombination test; --- Part 5. testicular cells UDS test; --- Part 6. mammalian cells in vitro sister chromatid exchange test; --- Part 7. mouse ear swelling test; Part --- Article 8.  fossa lymph node assay; --- Part 9. Determination of serum hemolysin test; Part --- Article 10. T lymphocyte proliferation assay test; --- Part 11. germline mutation assay; --- Part 12. single cell gel electrophoresis test; --- Part 13. fluorescence in situ hybridization; Part --- Article 14. SDS- polyacrylamide gel electrophoresis; --- Part 15. PCR-SSCP experiment; Part --- Article 16. Western-Blot test; --- Part 17. Behavior of mammalian toxicology tests; --- Part 18. Detection of DNA adducts; --- Part 19. NorthernBlot experiment; --- Part 20. Bradford protein assay; --- Part 21. agarose gel electrophoresis; --- Part 22. Tm value determination method of DNA; --- Part 23. organelle separation experimental methods; --- Part 24. cellular immunity in vitro assay method; --- Part 25. humoral immune function tests; --- Part 26. macrophage function tests; --- Part 27. Flow cytometry of apoptosis; --- Part 28. Application shuttle plasmid mutations Inspection; --- Part 29. Biochemical Oxygen Demand (BOD) was measured. This section SN/T Section 92497 of. The partial modification of the use of "Toxicology test methods and techniques" immune toxicology research method, technical content and the method described above Exactly the same, in a standard text format according to GB/T 1.1-2000 made editorial changes. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau. The main drafters of this section. Zhang Yuan, Wangli Bing, Wang Hua, Zhao Zhuo, in Chi Rui, Xiongzhong Jiang. The first part of the Department of Inspection and Quarantine issued by industry standards. Import and export of dangerous chemicals - Test methods Part 9. Determination of serum hemolysin test

1 Scope

SN/T 2497 provisions of this part of the hemolysin test measurement terms and definitions, test principle, test methods and test results. This section applies to half of hemolytic assay to detect hazardous chemicals import and export of sheep red blood cells of mice induced by hemolysin influences.

2 Normative references

The following documents contain provisions which, through reference SN/T 2497 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB 14924.1 animal feed common quality standards GB 14925 Laboratory Animal Facilities and Environment 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this section. 3.1.1 After SRBC immunization of animals to produce antibodies. 3.2 Acronyms The following abbreviations apply to this section. 3.2.1 SRBC

4 Test principle

With sheep red blood cells (SRBC) immunization of mice, and then the immune cells (mouse spleen B lymphocytes), SRBC and complement in liquid Phase in the reaction medium. IgM SRBC antibody-forming cells produced to form an antigen-antibody complex, activation of the complement system, SRBC lysis and the release of hemoglobin, spectrophotometer quantitatively. The measured values reflect the functional antibody-forming cells to produce antibodies.

5 Test methods

5.1 Test Animals Mice, weighing 18g ~ 25g. Experimental animal breeding conditions should be consistent with GB 14924.1 GB 14925 and related provisions. 5.2 Reagents and materials Preparation of 5.2.1 SRBC Defibration anti-coagulation treated SRBC, with NS washed twice, each time 2000r/min centrifugal 5min. The supernatant was discarded, made SRBC suspension ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 2497.9-2010_English be delivered?

Answer: Upon your order, we will start to translate SN/T 2497.9-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 2 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 2497.9-2010_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 2497.9-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.