SN/T 2497.23-2010 English PDF

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SN/T 2497.23-2010: Test method of import and export dangerous chemicals. Part 23: Separation of cellular organelles
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2497.23-2010	139	Add to Cart	2 days	Test method of import and export dangerous chemicals. Part 23: Separation of cellular organelles	Valid

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GB/T 35925   GB/T 4472   GB/T 3143   SN/T 2497.3   SN/T 2497.15   SN/T 2497.14   

Basic data

Standard ID: SN/T 2497.23-2010 (SN/T2497.23-2010)
Description (Translated English): Test method of import and export dangerous chemicals. Part 23: Separation of cellular organelles
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: G09
Classification of International Standard: 13.300
Word Count Estimation: 5,536
Date of Issue: 2010-03-02
Date of Implementation: 2010-09-16
Regulation (derived from): National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of dangerous chemicals test organelle separation test method, the test sample, the test solution and the test procedure. This standard applies to import and export of dangerous chemicals organelle separation test experimental methods.

SN/T 2497.23-2010: Test method of import and export dangerous chemicals. Part 23: Separation of cellular organelles

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of import and export dangerous chemicals.Part 23. Separation of cellular organelles Exit inspection and quarantine industry standard book People's Republic of China Import and export of dangerous chemicals - Test methods Part 23. organelle isolation test methods Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts. --- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test; --- Part 2. plaque-forming cells (PFC) test; --- Part 3. Large Zhuan reproduction test; --- Part 4. Saccharomyces cerevisiae mitotic recombination test; --- Part 5. testicular cells UDS test; --- Part 6. mammalian cells in vitro sister chromatid exchange test; --- Part 7. mouse ear swelling test; Part --- Article 8.  fossa lymph node assay; --- Part 9. Determination of serum hemolysin test; Part --- Article 10. T lymphocyte proliferation assay test; --- Part 11. germline mutation assay; --- Part 12. single cell gel electrophoresis test; --- Part 13. fluorescence in situ hybridization; Part --- Article 14. SDS- polyacrylamide gel electrophoresis; --- Part 15. PCR-SSCP experiment; Part --- Article 16. Western-Blot test; --- Part 17. Behavior of mammalian toxicology tests; --- Part 18. Detection of DNA adducts; --- Part 19. NorthernBlot experiment; --- Part 20. Bradford protein assay; --- Part 21. agarose gel electrophoresis; --- Part 22. Tm value determination method of DNA; --- Part 23. organelle separation experimental methods; --- Part 24. cellular immunity in vitro assay method; --- Part 25. humoral immune function tests; --- Part 26. macrophage function tests; --- Part 27. Flow cytometry of apoptosis; --- Part 28. Application shuttle plasmid mutations Inspection; --- Part 29. Biochemical Oxygen Demand (BOD) was measured. This is Part 23 SN/T 2497 series of standards. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau. The main drafters of this section. Lu Gang, Wangli Bing, Chi Yu Feng, Li Xue Yang, Zhao Qing Pang Zhen. The first part of the Department of Inspection and Quarantine issued by industry standards. Import and export of dangerous chemicals - Test methods Part 23. organelle isolation test methods

1 Scope

SN/T 2497 provisions of this part of the separation of experimental methods import and export of dangerous chemicals organelles test, the test sample, the test Test solutions and test procedures. This section applies to the import and export of dangerous chemicals isolated experimentally test organelles.

2 Terms and definitions

The following terms and definitions apply to this section. 2.1 Bilayer membrane organelles of eukaryotic cells, intracellular oxidative phosphorylation and ATP formed the main place. 2.2 When cells were homogenized crushed, after rupture of the membrane structure endomembrane system themselves up reclosable vesicles (mainly in the endoplasmic reticulum and Golgi Body), these vesicle diameter of about 100nm or so, are heterogeneous aggregates. 2.3 In the cell membrane surrounded by a tunnel system, it is an important cell organelles. Principle 3 The proportion of cells of different sizes and structures are not the same, the centrifugal sedimentation rate is not the same, according to this principle, by differential centrifugation, First with a low speed larger particles precipitate, and then a high speed, the float in the supernatant of the precipitated particles, so that a variety of cell structures, Such as the nucleus, mitochondria can be separated.

4 Reagents

4.1 NS 4.2 RSB solution Tris · Cl final concentration of 10mM; MgCl2 final concentration of 3mM; KCl to a final concentration of 10mM. The pH was adjusted with dilute hydrochloric acid To 7.4, after autoclaving, 4 ℃ preservation. The content of each component of the solution. D- sorbitol final concentration of 2M; HEPES at a final concentration of 10mM; EDTA to a final concentration 2mM. Value with dilute NaOH solution to adjust the pH to 7.4, membrane filtration sterilization, 4 ℃ preservation.

5 test procedure

5.1 cell lysis Cells were resuspended RSB solution, 1.10 volume ratio of 3% NP-40, repeatedly mixing, the ice bath was 10min. 5.2 Separation of nucleus and mitochondria In the ultracentrifuge, the cell lysate was 5000r/min centrifugal 20min, the supernatant was collected; ......

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