SN/T 2497.18-2010 English PDF

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SN/T 2497.18-2010: Test method of import and export dangerous chemicals. Part 18: Detection method of DNA adduct
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2497.18-2010	139	Add to Cart	2 days	Test method of import and export dangerous chemicals. Part 18: Detection method of DNA adduct	Valid

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GB/T 35925   GB/T 4472   GB/T 3143   SN/T 2497.3   SN/T 2497.11   SN/T 2497.10   

Basic data

Standard ID: SN/T 2497.18-2010 (SN/T2497.18-2010)
Description (Translated English): Test method of import and export dangerous chemicals. Part 18: Detection method of DNA adduct
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: G09
Classification of International Standard: 13.300
Word Count Estimation: 5,526
Date of Issue: 2010-03-02
Date of Implementation: 2010-09-16
Regulation (derived from): National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of dangerous chemicals DNA adduct detection methods Reagents and materials, the main equipment, test procedures and test results. This standard applies to 32P labeled assay after import of dangerous chemicals induced DNA adducts.

SN/T 2497.18-2010: Test method of import and export dangerous chemicals. Part 18: Detection method of DNA adduct

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of import and export dangerous chemicals.Part 18. Detection method of DNA adduct Exit inspection and quarantine industry standard book People's Republic of China Import and export of dangerous chemicals - Test methods Part 18. Detection of DNA adducts Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts. --- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test; --- Part 2. plaque-forming cells (PFC) test; --- Part 3. Large Zhuan reproduction test; --- Part 4. Saccharomyces cerevisiae mitotic recombination test; --- Part 5. testicular cells UDS test; --- Part 6. mammalian cells in vitro sister chromatid exchange test; --- Part 7. mouse ear swelling test; Part --- Article 8.  fossa lymph node assay; --- Part 9. Determination of serum hemolysin test; Part --- Article 10. T lymphocyte proliferation assay test; --- Part 11. germline mutation assay; --- Part 12. single cell gel electrophoresis test; --- Part 13. fluorescence in situ hybridization; Part --- Article 14. SDS- polyacrylamide gel electrophoresis; --- Part 15. PCR-SSCP experiment; Part --- Article 16. Western-Blot test; --- Part 17. Behavior of mammalian toxicology tests; --- Part 18. Detection of DNA adducts; --- Part 19. NorthernBlot experiment; --- Part 20. Bradford protein assay; --- Part 21. agarose gel electrophoresis; --- Part 22. Tm value determination method of DNA; --- Part 23. organelle separation experimental methods; --- Part 24. cellular immunity in vitro assay method; --- Part 25. humoral immune function tests; --- Part 26. macrophage function tests; --- Part 27. Flow cytometry of apoptosis; --- Part 28. Application shuttle plasmid mutations Inspection; --- Part 29. Biochemical Oxygen Demand (BOD) was measured. This is Part 18 SN/T 2497 series of standards. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau. The main drafters of this section. to Yanjun, Wangli Bing, Zhao Haolibao, Zhao Lihua, Han Wei, Xiong Zhongjiang. The first part of the Department of Inspection and Quarantine issued by industry standards. Import and export of dangerous chemicals - Test methods Part 18. Detection of DNA adducts

1 Scope

SN/T 2497 provisions of this part of the main reagents and materials import and export of hazardous chemicals DNA adduct detection methods, the main instrument Device, test procedures and test results. This section applies to post-32P labeled DNA adducts were detected due to the import and export of hazardous chemicals. 2 terms, definitions and abbreviations 2.1 Terms and Definitions The following terms and definitions apply to this section. 2.1.1 DNA adduct compound is covalently linked to the electron affinity of the compound or its metabolites with DNA, DNA is the chemical damage One of the main forms of injury. 2.2 Acronyms The following abbreviations apply to this section. 2.2.1 DNA deoxyribonucleic acid Principle 3 Strand DNA adducts containing nucleic acid in the exonuclease action degrades the 3 'nucleoside monophosphates, (Pl or S1) treated with nuclease 3 'monophosphate nucleosides, the normal 3' nucleotide monophosphate is dephosphorylation and 3 'monophosphate nucleoside adducts can resist having this role. in T4 polynucleotide kinase under the action of specific, 32P-ATP phosphate groups transferred to a 5 'hydroxyl terminus of a nucleotide adduct, Forming 3'-5 'diphosphate nucleosides, quantified by detecting the amount of 32P-ATP to DNA damage resulting adduct.

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade, sterile distilled water was used or ultrapure water. Endonuclease 4.1. 4.2 exonuclease. 4.3 P1 nuclease. 4.4 32P-ATP. 4.5 T4 polynucleotide kinase. 4.6 T4 polynucleotide Kinase Buffer. 0.1mol/LBicine-NaOH, 0.1mol/LMgCl2,0.1mol/L-threitol disulfide, 10mmol/L spermidine, pH value of 9.5. 4.7 ODS-TLC plate (reverse phase adsorption TLC plate). 4.8 PEI-TLC plate (TLC plate anion exchange).

5 Key Equipment

5.1 UV spectrophotometer. ......

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