SN/T 2452-2010 English PDF

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SN/T 2452-2010: Protocol of the quarantine for sheep pox and goat pox
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2452-2010	369	Add to Cart	3 days	Protocol of the quarantine for sheep pox and goat pox	Valid

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Basic data

Standard ID: SN/T 2452-2010 (SN/T2452-2010)
Description (Translated English): Protocol of the quarantine for sheep pox and goat pox
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,172
Date of Issue: 2010-01-10
Date of Implementation: 2010-07-16
Regulation (derived from): National Quality Inspection [2010] No. 13
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the sheep and goat pox epidemiology, clinical symptoms and pathological examination, laboratory testing and other technical content. This standard applies to sheep and goat pox diagnosis, quarantine.

SN/T 2452-2010: Protocol of the quarantine for sheep pox and goat pox

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the quarantine for sheep pox and goat pox Exit inspection and quarantine industry standard book People's Republic of China Sheep and goat pox quarantine Technical Specifications Issued on. 2010-01-10 2010-07-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

The standard reference OIE "Terrestrial animal diagnostic tests and vaccines Standards Manual" (2008) developed the first chapter 2.7.14. The Standard Appendix A, Appendix B, Appendix C and Appendix D are normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Inner Mongolia People's Republic of China Exit Inspection and Quarantine, Beijing People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine, Xinjiang People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Wang Haiyan, Liu School, Zhaolin Li, Liu Hong, Ao Wei Hua, Gao Zhiqiang, a new season, Xue Qiang, Qiao Caixia, Ren Lixin. This standard is the first release of the entry-exit inspection and quarantine industry standards. Sheep and goat pox quarantine Technical Specifications

1 Scope

This standard specifies the sheep and goat pox epidemiological investigation, the technical content of the clinical symptoms and pathological examination, laboratory testing and so on. This standard applies to sheep and goat pox diagnosis, quarantine.

2 Equipment and Materials

2.1 incubator, constant temperature water bath, refrigerator. 2.2 Biological microscopy, transmission electron microscopy. 2.3 autoclaves, high-speed refrigerated centrifuge. 2.4 Single-channel micro pipette. 1μL ~ 10μL, 100μL, 200μL, 1000μL. 2.5 cell culture flasks. 25cm2. 2.6 Reader, protein gel electrophoresis, power transfer device.

3 media and reagents

3.1 Hank's solution. See Appendix A A. Chapter 1. 3.2 0.25% trypsin digestion. See Appendix A, Section A. 2. 3.3 MEM cell culture medium. The specific method of preparation carried out according to the procedure specified by the manufacturer, with a 0.2μm membrane filter sterilized, packed in small Bottle, 4 ℃ stored for use. 3.4 EDTA- trypsin dispersion. See Appendix A, Section A. 3. 3.5 Antimicrobial Solution. See Appendix A, Section A. 4. 3.6 Sheep and goat pox antigen negative serum and the appropriate standards and the standard positive serum. 3.7 penicillin, streptomycin, nystatin (or amphotericin), neomycin. 3.8 PBS solution. See Appendix A, Section A. 5. 3.9 10 × Tris- glycine electrophoresis buffer solution (pH8.3). See Appendix A, section A. 6. 3.10 Transfer Buffer Solution. See Appendix A, Section A. 7. 3.11 nitrocellulose membrane, fetal bovine serum, protein molecular weight Marker, bovine serum albumin, horseradish peroxidase-labeled rabbit (or small Mouse) anti-goat IgG. 3.12 12% separating gel. See Appendix A. 8. 3.13 5% laminated plastic. See Appendix A, Section A. 9. 3.14 1 × SDS gel loading buffer. See Appendix A, Section A. Chapter 10. 3.15 Coomassie blue staining solution. See Appendix A, Section A. 11. 3.16 1% phosphotungstic acid solution (pH7.2). See Appendix A, section A. Chapter 12. 3.17 agar plates. See Appendix A, Section A. 13. 3.18 Substrate Solution. See Appendix A, Section A. 14. 3.19 hematoxylin staining solution. See Appendix A, Section A. 15. 3.20 0.5% eosin solution. See Appendix A, Section A. Chapter 16. 3.21 ethanol, xylene. 3.22 Paraffin. mp were 52 ℃ ~ 54 ℃, 54 ℃ ~ 56 ℃, 56 ℃ ~ 58 ℃. Preparation of 3.23 lambs testicular cells. Appendix B. ......

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