SN/T 2345-2009 English PDF

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SN/T 2345-2009: The pine resin canker quarantine and identification methods
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2345-2009	319	Add to Cart	3 days	The pine resin canker quarantine and identification methods	Valid

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Basic data

Standard ID: SN/T 2345-2009 (SN/T2345-2009)
Description (Translated English): The pine resin canker quarantine and identification methods
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 12,144
Date of Issue: 2009-07-07
Date of Implementation: 2010-01-16
Regulation (derived from): National Quality Inspection [2009] No. 290
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the plant quarantine in the pine resin canker (Fusarium circinatum Nircnberg and O "Donnell) quarantine identification. This standard applies from pine resin canker in the countries and regions Pinus (Pinus spp.), Douglas fir is (Pseudotsuga spp.) and other pine resin canker host plants, seeds, seedlings and timber entry quarantine and identification.

SN/T 2345-2009: The pine resin canker quarantine and identification methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The pine resin canker quarantine and identification methods Exit inspection and quarantine industry standard book People's Republic of China Pine resin canker quarantine and identification methods Posted 2009-07-07 2010-01-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

The Standard Appendix A, Appendix B, Appendix C, Appendix D and Appendix E are informative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted by the People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Baisheng, Liaotai Lin, Ji Rui, Wu Cuiping, Wu Xinhua, Ann Yulin. This standard is the first release of the entry-exit inspection and quarantine industry standards. Pine resin canker quarantine and identification methods

1 Scope

Identification methods. spp. ) Pine resin canker and other host plants of quarantine and identification of entry of seeds, seedlings and timber. Principle 2 2.1 taxonomic status Fungi (Fungi), imperfect fungi Amon (Deuteromycotina), Trichosporon Gang (Hyphomycetes), tumor seat spore head (Tuberculariales), 2.2 Host Body, cone, seed and soil-borne. National and regional distribution see Appendix A. 2.3 Identification of principle Pine resin canker form (see Appendix B Figure B.1, Figure B.2), host range, transmission, Damage symptoms and molecular biology Characteristics of the bacteria is the main basis for quarantine and identification of. 3 instruments, appliances and reagents 3.1 equipment, appliances --- PCR instrument; --- Gel electrophoresis equipment; --- Centrifuge; --- Vortex device; --- Constant temperature water bath; --- Gel imaging system; --- Other conventional laboratory equipment, apparatus. 3.2 Reagents Sucrose, dextrose, agar, peptone, potassium dihydrogen phosphate, magnesium sulfate (MgSO4 · 7H2O), quintozene (PCNB), Shigeru scattered kill Agents, triadimefon, Ampicillin, rifampicin, Tris (Tris), cetyltrimethylammonium bromide (of CTAB), ethylenediaminetetraacetic Acetic acid (EDTA), sodium hypochlorite, ethanol, proteinase K, phenol, chloroform, isoamyl alcohol, ammonium acetate (NH4Ac), magnesium chloride (Of MgCl2) dimethyl sulfoxide (DMSO), dNTP, T Rao q DNA polymerase, ethidium bromide (the EB), liquid nitrogen.

4 identification of quarantine

4.1 Site Inspection Check the wood, sheet metal or seedlings surface, with or without flow fat, ulcer plaque. With an ax, or a saw blade, respectively horizontal, vertical incision, observe timber ......

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