SN/T 2344-2014 English PDFSN/T 2344: Historical versions
Basic dataStandard ID: SN/T 2344-2014 (SN/T2344-2014)Description (Translated English): Detection and identification of cucumber green mottle mosaic virus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 07.080 Word Count Estimation: 16,134 Date of Issue: 12/1/2014 Date of Implementation: 5/1/2015 Older Standard (superseded by this standard): SN/T 2344-2009 Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the molecular biology and immunology methods cucumber green mottle mosaic virus quarantine identified. This standard applies to seed of a plant quarantine and identification possible with cucumber green mottle mosaic virus, seedlin SN/T 2344-2014: Detection and identification of cucumber green mottle mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Cucumber green mottle mosaic virus quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 2344-2009 Cucumber green mottle mosaic virus quarantine and identification methods Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces the SN/T 2344-2009 "cucumber green mottle mosaic virus quarantine and identification methods." This standard compared with SN/T 2344-2009, the main technical changes are as follows. Added "real-time quantitative RT-PCR method" and the "rapid immunochromatographic test." This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Beijing People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chen Qing, Liang Miao, Huang Feng, Liao Furong, Chen fine red, Chen Yun, Lin Shiming. This standard replaces the standards previously issued as follows. --- SN/T 2344-2009. Cucumber green mottle mosaic virus quarantine and identification methods1 ScopeThis standard specifies the molecular biology and immunology methods cucumber green mottle mosaic virus quarantine identified. This standard applies to seed of a plant quarantine and identification possible with cucumber green mottle mosaic virus, seedlings and plant products.2 cucumber green mottle mosaic virus Basic InformationName. cucumbergreenmottlemosaicvirus Abbreviation. CGMMV Taxonomic position. tobacco mosaic virus genus (Tobamovirus) members CGMMV by mechanical transmission, but also by seed dispersal. Grafting and other farming operations is one of the main factors of disease epidemics in the field, CGMMV also been Cuscutasubinclusa, hops dodder (C.lupuliformis) and C.campestris mass poisoning, a plant Once the disease, if not carried out the killing process, soil can also be carriers of the virus, infected soil can also become the source of infection of disease occurrence. For more information, see Appendix A.3 PRINCIPLE OF THE METHODAccording to a specific reaction of cucumber green mottle mosaic virus antibody between the blades of melon seeds or ELISA test; the basis of the Characteristics of the viral genome-specific PCR testing, the results determined by electrophoretic bands size.4 Equipment and Reagents4.1 equipment and appliances Trace Mills, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR instrument, electrophoresis, horizontal electrophoresis tank gel imager, Desktop high-speed refrigerated centrifuge, water bath, pH meter, pipettes (1000μL, 200μL, 20μL, 2μL), 96 well microtiter plates, mortar or the like; anti Insect greenhouse. 4.2 Reagents Reagents and buffers see Appendix B, Appendix C, Appendix D, Appendix E, Appendix F, Appendix G. Preparation of the test sample 5 5.1 Preparation of seed samples Picked malformation, immature seeds were sown in sterilized soil, grow to be 3 to 4 leaves of plants will show symptoms numbers do not show symptoms of the plant Strain grouping (10 per group) and numbered, collected leaves into 2 parts, respectively, based on the need for follow-up tests. Can also be picked malformation, immature seeds directly enzyme-linked assays and molecular biology. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2344-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 2344-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 2344-2014_English with my colleagues?Answer: Yes. 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