SN/T 2342.2-2010 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2342.2-2010	359	Add to Cart	3 days	Detection and identification of apple fruit crinkle viroid	Obsolete

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Basic data

Standard ID: SN/T 2342.2-2010 (SN/T2342.2-2010)
Description (Translated English): Detection and identification of apple fruit crinkle viroid
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 9,972
Date of Issue: 2010-11-01
Date of Implementation: 2011-05-01
Regulation (derived from): National Quality Inspection (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the wrinkled apple fruit quarantine and identification of the virus. This standard applies to apples with viral entry wrinkled fruit seedlings or other propagating materials gun epidemic and identification.

SN/T 2342.2-2010: Detection and identification of apple fruit crinkle viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of apple fruit crinkle viroid People's Republic of China Entry-Exit Inspection and Quarantine Standards Apple fruit crinkle viroid identification of quarantine Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2342 standard is divided into two parts. --- Apple stem grooving virus quarantine and identification methods; --- Apple fruit crinkle viroid quarantine and identification methods. This section SN/T 2342 Part 2. This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section drafted by. China Inspection and Quarantine Science Research Institute. The main drafters of this section. MATURAL Weimei Sheng, Zhang Yongjiang, Ma Jie, Xiangning, Li Mingfu. Apple fruit crinkle viroid identification of quarantine

1 Scope

This section SN/T 2342 provisions Apple fruit crinkle viroid quarantine and identification methods. This section applies to quarantine and identification with Apple fruit crinkle viroid entry seedlings or other propagating material. Principle 2 Apple fruit crinkle viroid Name. Applefruitcrinkleviroid, Abbreviation. AFCVd. Apple fruit crinkle viroid feature is present standards The main basis; taxonomic status of such virus, host range, disease symptoms, geographical distribution, transmission, and other genomic features, see Appendix A. 3 instruments, appliances and reagents 3.1 Instrument 3.1.1 PCR amplification. 3.1.2 electrophoresis, electrophoresis tank. 3.1.3 water bath. 3.1.4 High-speed refrigerated centrifuge. 3.1.5 gel imaging system. 3.1.6 oscillator. 3.1.7 fridge. 3.1.8 electronic analytical balance (0.001g). 3.2 Appliances 3.2.1 adjustable pipette (0.1μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 100μL ~ 1000μL). 3.2.2 adjustable pipette tips. 3.2.3 centrifuge tube. 3.2.4 mortar. 3.3 Reagents 3.3.1 RNA extraction buffer 20mmol/L Tris-HCl, pH8.0 10mmol/L disodium ethylenediaminetetraacetate (C10H14N2O8Na2 · 2H2O) 0.8mol/L sodium chloride (NaCl) 1% sodium dodecyl sulfate (SDS) 0.5% diethyl dithiocarbamate (DIEC A) 2% polyvinyl pyrrolidone (PVP, MV24000-40000) 0.5% mercaptoethanol ......

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