SN/T 2131.1-2008 English PDF
Basic dataStandard ID: SN/T 2131.1-2008 (SN/T2131.1-2008)Description (Translated English): Determination of DSP in shellfish for import and export. Part 1: Inhibition method of fluorescence phophatase activity Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X20 Classification of International Standard: 67.050 Word Count Estimation: 14,141 Date of Issue: 2008-09-04 Date of Implementation: 2009-03-16 Regulation (derived from): Industry standard filing Notice No. 11 of 2008 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the quantitative detection of diarrhetic shellfish toxins in shellfish fluorescent phosphatase inhibition test methods. This standard applies to bivalve shellfish meat, shellfish column of okadaic acid and its derivatives test. SN/T 2131.1-2008: Determination of DSP in shellfish for import and export. Part 1: Inhibition method of fluorescence phophatase activity---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of DSP in shellfish for import and export.Part 1. Inhibition method of fluorescence phophatase activity Exit inspection and quarantine industry standard book People's Republic of China Importers and shellfish diarrhea shellfish toxin detection method Part 1. Fluorescent phosphatase inhibition assay Posted 2008-09-04 2009-03-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis section is SN/T 2131 Part 1. This section of Appendix A normative appendix. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this section. Wu Bin, Li Ye, Wang Yuping, LI Zhen-Rong, Xie Yan. The first part of the Department of Inspection and Quarantine issued by industry standards. Importers and shellfish diarrhea shellfish toxin detection method Part 1. Fluorescent phosphatase inhibition assay1 ScopeThis section SN/T 2131 provides for the quantitative detection of shellfish toxins in shellfish diarrhea fluorescence phosphatase inhibition test. This section applies to test bivalve shellfish meat, shellfish column of okadaic acid and its derivatives.2 AcronymsThe following abbreviations apply to this part of the SN/T 2131's. 2.1 Diarrhea shellfish toxins. 2.2 Okadaic acid. 2.3 DTX Ju Okadaic acid derivative family.3 Sample preparation and preservationSave 3.1 sample Such as laboratory tests can not immediately label should indicate the source, respectively, sampling dates, the variety and number, put a plastic bag sealed. After sampling the sample Product should be immediately frozen at less -18 ℃. 3.2 Preparation of the sample Wash thoroughly with water to be tested shell, open shell, thoroughly clean the interior shell, remove the internal waste quality, remove all shellfish tissue, with a filter Paper try to dry the excess water shellfish tissue to be tested shellfish organization may not be less than 50g.4 Determination4.1 Method summary Measurement principle of this method is okadaic acid (Okadaicacid, OA) and its toxic derivatives (DTXs) and their ability to inhibit serine Acid and protein phosphatase activity of threonine directly related, in particular, PP1 and PP2A two proteins; phosphatase enzyme and a fluorescent substance in Microplate experiments, the concentration of okadaic acid and its derivatives directly determine the ability of phosphatase enzyme hydrolysis of a fluorescent substance, after hydrolysis Luciferase substrates fluorescence and fluorescence is read by a fluorescence microplate reader, the concentration of the toxin in the sample can be obtained by standard curve meter Operators draw. 4.2 Reagents and materials Unless otherwise specified, the reagents were of analytical grade, water is deionized water. 4.2.1 100% (by volume) of methanol. 4.2.2 2.5mol/L sodium hydroxide solution. 100g of sodium hydroxide dissolved in 500mL of water, dilute to 1000mL. 4.2.3 2.5mol/L hydrochloric acid solution of. 205mL concentration of 37% (volume fraction) of hydrochloric acid was added 400mL of water volume ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2131.1-2008_English be delivered?Answer: Upon your order, we will start to translate SN/T 2131.1-2008_English as soon as possible, and keep you informed of the progress. 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