SN/T 2121-2014 English PDFUS$419.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2121-2014: Quarantine protocol for epizootic haematopoietic necrosis Status: Valid SN/T 2121: Historical versions
Basic dataStandard ID: SN/T 2121-2014 (SN/T2121-2014)Description (Translated English): Quarantine protocol for epizootic haematopoietic necrosis Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 07.080 Word Count Estimation: 16,116 Date of Issue: 12/1/2014 Date of Implementation: 5/1/2015 Older Standard (superseded by this standard): SN/T 2121-2008 Quoted Standard: GB/T 6682; GB/T 18088; GB 19489 Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the Epizootic haematopoietic necrosis clinical symptoms, pathogen isolation, ELISA, polymerase chain reaction and detection methods of indirect immunoperoxidase. This standard applies to Epizootic haematopoietic necrosis diagnosis, SN/T 2121-2014: Quarantine protocol for epizootic haematopoietic necrosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Quarantine protocol for epizootic haematopoietic necrosis People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Replace SN/T 2121-2008 Technical specification for epidemic hematopoietic organ necrosis Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 2121-2008 "Practical Technical Specification for Epidemic Hematopoietic Organ Necrosis". The main technical differences between this standard and SN/T 2121-2008 are as follows. --- Increased description of clinical symptoms; --- Increased indirect immunoperoxidase method; --- Modified the comprehensive judgment. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Chinese People Shandong Entry-Exit Inspection and Quarantine Bureau of the Republic. The main drafters of this standard. Liu Wei, Jia Peng, Zheng Xiaocong, He Junqiang, Ma Xiaowen, Wang Jinjin, Yu Li, Shi Xiujie, Lan Wensheng, Chen Bing, Wang Hongying. The previous versions of the standards replaced by this standard are ---SN/T 2121-2008. Technical specification for epidemic hematopoietic organ necrosis1 ScopeThis standard specifies the clinical symptoms of epidemic hematopoietic organ necrosis, pathogen isolation, enzyme-linked immunosorbent assay, polymerase chain reaction and Indirect immunoperoxidase assay. This standard applies to the diagnosis, detection and monitoring of epidemic hematopoietic organ necrosis.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 18088 entry and exit animal quarantine sampling GB 19489 General requirements for laboratory biosafety3 OverviewEpidemic hematopoietic necrosis (Epizotic haematopoieticnecrosis, EHN) is an epidemic hematopoietic necrosis Epizotichaematopoieticnecrosisvirus (EHNV) causes finfish to be characterized by systemic clinical or subclinical infections Viral infections (see Appendix A).4 reagents and materials4.1 Water. Meet the specifications of the first grade water in GB/T 6682. 4.2 EHNV reference strain. provided by the designated unit. 4.3 Cell line. Blue scorpion fibroblast cell line (Bluegilfry, BF-2), Fathead 鱥 cell line (Fathead Minnow, FHM), Epithelioma Papulosum Cyprini (EPC) or Large Scale Salmon Embryo Cell Line (ChinookSalmonEmbryo, CHSE-214). 4.4 4-Hydroxyethylpiperazineethanesulfonic acid (HEPES). 4.5 Rabbit anti-EHNV antibody or murine anti-EHNV antibody. supplied by the designated unit. 4.6 Horseradish peroxidase-labeled anti-rabbit or anti-mouse immunoglobulin (IgG). 4.7 TaqDNA enzyme. Store at -20 °C to avoid drastic changes in temperature. 4.8 dNTPs. containing 10 mmol/L of dCTP, dGTP, dATP and dTTP. 4.9 Primers. The concentration was 40 μmol/L. The sequence is as follows. M151. 5'-AACCCGGCTTTCGGGCAGCA-3' M152. 5'-CGGGGCGGGGTTGATGAGAT-3' A 321 bp fragment of the major capsid protein gene of EHNV was amplified. M153. 5'-ATGACCGTCGCCCTCATCAC-3' ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2121-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 2121-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 2121-2014_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 2121-2014_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |