SN/T 2101-2016 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2101-2016: Determination of Mycobacterium tuberculosis in milk and dairy products for export - Real-time PCR method Status: Valid SN/T 2101: Historical versions
Basic dataStandard ID: SN/T 2101-2016 (SN/T2101-2016)Description (Translated English): Determination of Mycobacterium tuberculosis in milk and dairy products for export - Real-time PCR method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X16 Classification of International Standard: 67.100.01 Word Count Estimation: 10,128 Date of Issue: 3/9/2016 Date of Implementation: 2016-10-01 Older Standard (superseded by this standard): SN/T 2101-2008 Regulation (derived from): [2016] 131 State Quality Inspection Issuing agency(ies): General Administration of Customs SN/T 2101-2008: Determination of Mycobacterium tuberculosis for milk and milk products. Real-time PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of Mycobacterium tuberculosis for milk and milk products.Real-time PCR method Exit inspection and quarantine industry standard book People's Republic of China Milk and dairy products Mycobacterium tuberculosis detection methods in Fluorescence quantitative PCR Posted 2008-07-17 2009-02-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordAppendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Woo, Huchuan Wei, Jia, Li Ye, LI Zhen-Rong, Sun Yingjie?. This is the first issue of inspection and quarantine industry standards. Milk and dairy products Mycobacterium tuberculosis detection methods in Fluorescence quantitative PCR1 ScopeThis standard specifies the type of milk and dairy products of bovine tuberculosis and human-type operation method of Mycobacterium tuberculosis PCR for detection. This standard applies to milk, milk powder Mycobacterium tuberculosis detection, other dairy products may refer to. Principle 2 Milk and Mycobacterium tuberculosis PCR assay method is the use of dairy products in the classic TaqMan probe technology. According to bovine and human Conserved sequence of Mycobacterium tuberculosis to design a pair of primers and a dual-labeled probes specific fluorescent probes 5 'and 3' end points Do not use FAM and TAMRA fluorescence-labeled. When the PCR reaction annealing, the primers and probes simultaneously with the target gene fragment binding at this time Report on the probe fluorophore FAM fluorescence signal emitted by the fluorophore is quenched by TAMRA absorption instrument undetectable FAM group Mission fluorescence signal emitted; when the PCR reaction is carried out to extend, T Rao q enzyme under the guidance of primer to four nucleotides as a substrate, according to the base Base pairing principle, the synthesis of new strand along the template strand, when carried to the probe binding sites, the hindered probes can not continue at this time T Rao q enzyme plays its 5 '→ 3' exonuclease function of the probe into the single nucleotide extension in order to continue to complete the process, FAM group And TAMRA groups are free in solution, the instrument can detect the fluorescence signal emitted by FAM group.3 AbbreviationsThe following abbreviations apply to this standard. 3.1 Fluorescent PCR Fluorescent polymerase chain reaction. 3.2 When the number of cycles of each reaction tube fluorescent signal reaches the set value illustrates experienced. 3.3 DNA DNA. 3.4 T Rao enzyme q T Rao q DNA polymerase.4 Materials and Reagents4.1 Instruments and Equipment 4.1.1 PCR fluorescence detector. 4.1.2 high-speed desktop refrigerated centrifuge. 4.1.3 ordinary tabletop centrifuge. 4.1.4 Vortex. 4.1.5 Refrigerator. 4 ℃ ± 1 ℃, -20 ℃ ± 2 ℃. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2101-2016_English be delivered?Answer: Upon your order, we will start to translate SN/T 2101-2016_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version SN/T 2101-2016?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 2101-2016 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
