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SN/T 2074-2008: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi
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SN/T 2074-2008: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi

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Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi Exit inspection and quarantine industry standard book People's Republic of China Genetically Modified qualitative Edible Fungus PCR detection method Posted 2008-04-29 2008-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine. The main drafters of this standard. Chenwen Bing, Shaobi Ying, Jiang Shuxun, Lishou Song, Zhu Xiaonan, Guo Lixin, Yang Jie. This standard is the first release of the entry-exit inspection and quarantine industry standards. Genetically Modified qualitative Edible Fungus PCR detection method

1 Scope

This standard specifies the sampling, sample preparation, qualitative PCR detection methods in the Mushroom GMO testing. This standard applies to the major edible fungus (mushroom, red mushroom, Agaricus blazei, mushrooms, mushroom, Boletus, Coprinus, mushroom, Agaricus bisporus, Pork bellies mushroom, Pleurotus, small mushroom, oyster mushrooms, Hericium, Pleurotus, Agrocybe, bamboo fungus, black fungus, white fungus, etc.) in the GMO Testing.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987) SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods

3 Abbreviations

The following abbreviations apply to this standard. 3.1 Derived from the Agrobacterium tumefaciens nopaline synthase gene. 3.2 3.3 β- glucuronidase gene (as a plant gene expression of a reporter gene). 3.4 Neomycin from E. coli K12 strain 3'-phosphotransferase Ⅱ genes. 3.5 Coding 18SrRNA (ribosomal) of DNA. Principle 4 After extraction of the sample DNA, for common exogenous gene transgenic edible fungi of the introduced gene sequence primers PCR Technology, DNA fragments of exogenous gene was specifically amplified, according to the experimental results, it is determined whether a sample containing the edible fungus exogenous gene ingredient. ...