SN/T 2010-2007 English PDFUS$389.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2010-2007: Method for elimination of phytoplasma Status: Valid
Basic dataStandard ID: SN/T 2010-2007 (SN/T2010-2007)Description (Translated English): Method for elimination of phytoplasma Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B21 Classification of International Standard: 65.020.20 Word Count Estimation: 12,144 Date of Issue: 2007-12-24 Date of Implementation: 2008-07-01 Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the plants and seedlings, bulbs and tissue culture scales phytoplasma removal and testing of the basic principles and methods. This standard applies to export plant seedlings, bulbs and tissue culture scales phytoplasmas removal and detection. SN/T 2010-2007: Method for elimination of phytoplasma---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Method for elimination of phytoplasma Exit inspection and quarantine industry standard book People's Republic of China Phytoplasma Removal Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThe Standard Appendix A, Appendix B, Appendix C, Appendix D are normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard by the People's Republic of China Shanghai Exit Inspection and Quarantine is responsible for drafting, Chinese Academy of Inspection and Quarantine participated in the drafting. The main drafters of this standard. Yang Cuiyun at Chui, Weimei Sheng, Zhao Wenjun, Qiao Yanyan, Tao Tingdian. This standard is the first release of the entry-exit inspection and quarantine industry standards. Phytoplasma Removal1 ScopeThis standard specifies the basic principles and methods for plant seedlings, bulbs and scales plantlets phytoplasma detection and removal. This standard applies to export plant seedlings, bulbs and scales plantlets phytoplasma detection and removal.2 Terms and definitionsThe following terms and definitions apply to this standard. 2.1 Parasitic on plant phloem and mediator insects, with no three-unit membrane cell wall structure of prokaryotes. Usually caused by plant Yellow and developmental abnormalities and other symptoms were leaves on the tetracycline family antibiotics sensitive, and not sensitive to penicillin. 2.2 Material for the plant tissue culture known as explants, the main form of organs, embryos, single cells and protoplasts and the like. 2.3 Application of Tissue Culture techniques to obtain regenerated shoots, by testing to confirm without harmful phytoplasma this plant can be regarded as de Phytoplasma seedlings. Principle 3 Phytoplasma forms generally spherical, oval, long rod-shaped, spindle-shaped strip and polymorphism irregular shape, the size of 50nm ~ 1000nm, which Virus structure is more complex, with a cell structure, but no cell wall, wrap unit is the unit film is composed of two layers of membrane proteins and an intermediate layer of lipid membrane Composition, a thickness of about 10nm. Phytoplasma in plants are mainly distributed in phloem, and apical bud tip meristem phytoplasma content of less or no phytoplasma. in High temperatures can be passivated phytoplasma, significantly reduced or suppressed reproduction phytoplasma in Plants. Phytoplasma tetracycline family antibiotics sensitive to Not sensitive to penicillin. 4 equipment, appliances, and reagents 4.1 Equipment Clean table, light incubator, oven, electronic balance, microwave. 4.2 Appliances Tweezers, scissors, alcohol lamp. 4.3 Reagents Unless otherwise specified, all reagents were of analytical grade. Alcohol, bleach, mercuric chloride, MS medium reagent (see Appendix A).5 phytoplasma Removal5.1 Removal of the shoot tip culture phytoplasma 5.1.1 Training material sterilization The tissue culture material rinse with running water, as well as irregularities in the gap at the scales with a soft brush to thoroughly clean the dirt, most ...... |
