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SN/T 1996-2007 English PDF

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1996-2007479 Add to Cart 3 days Detemination of diarrhetic shellfish poison in shellfish. ELISA method Obsolete

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Basic data

Standard ID: SN/T 1996-2007 (SN/T1996-2007)
Description (Translated English): Detemination of diarrhetic shellfish poison in shellfish. ELISA method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X20
Classification of International Standard: 07.100.30
Word Count Estimation: 12,131
Date of Issue: 2007-12-24
Date of Implementation: 2008-07-01
Quoted Standard: SN 0294
Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the diarrhetic shellfish toxins in shellfish sampling, sample preparation and enzyme-linked immunosorbent assay. This standard applies to edible parts of bivalve seafood diarrheal shellfish toxin testing.

SN/T 1996-2007: Detemination of diarrhetic shellfish poison in shellfish. ELISA method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detemination of diarrhetic shellfish poison in shellfish.ELISA method Exit inspection and quarantine industry standard book People's Republic of China Diarrhea shellfish shellfish poison test methods Enzyme-linked immunosorbent assay Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. the soldiers, Cao Juan occasion, Gao light, linen Lidan, Jindong right, Xie Yan, Li Li, Sun Jie. This is the first issue of inspection and quarantine industry standards. Diarrhea shellfish shellfish poison test methods Enzyme-linked immunosorbent assay

1 Scope

This standard specifies the sampling, sample preparation and method of enzyme-linked immunoassay diarrhea shellfish toxin testing. This standard applies to edible portions of bivalve seafood diarrhea Shellfish Toxin test.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN 0294 export shellfish shellfish toxin testing method diarrhea 3 definitions, terms and abbreviations 3.1 Definitions and Terminology The following terms and definitions apply to this standard. 3.1.1 Chemical structure okadaic acid (okadaic, acid, referred to as OA) and its derivatives represented after ingestion can produce diarrhea as The main features present in the shellfish marine life in general toxic substances. 3.2 Acronyms 3.2.1 Diarrhea shellfish toxins. 3.2.2 Enzyme-linked immunosorbent assay.

4 Sampling and sample preparation

Referring SN 0294 regulations.

5 measurement method

5.1 Principle of Measurement Determination of the basis of this method is a competitive enzyme immunoassay, free of diarrhea and diarrheal shellfish poisons shellfish toxin enzyme markers competition Fight diarrhea shellfish toxin antibody is not bound enzyme label is removed in the washing step. The enzyme substrate and chromogenic agent is added to the wells And incubation, the bound enzyme markers will be colorless chromogenic agent into a blue product was added to the reaction solution after the termination of the color change from blue to yellow color. Measuring the absorbance of the solution in the pores of 450nm wavelength microplate reader, the sample solution and diarrheal shellfish poisons into absorbance Inversely, drawn by the quantitative calibration curve. 5.2 Reagents and materials Unless otherwise specified, all chemical reagents were of analytical grade, water re-distilled water.
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