SN/T 1966-2007 English PDF
Basic dataStandard ID: SN/T 1966-2007 (SN/T1966-2007)Description (Translated English): Determination of chloramphenicol residues in aquatic product. Radio-receptor assay method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X20 Classification of International Standard: 67.050 Word Count Estimation: 12,171 Date of Issue: 2007-08-06 Date of Implementation: 2008-03-01 Quoted Standard: GB/T 6682 Regulation (derived from): Accredidation-Technology-Letter [2015] No. 247 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the determination of chloramphenicol residues in aquatic products sample preparation and analysis of radiation receptor (Charm ��) method. This standard applies to chloramphenicol residues in aquatic products screening assays. SN/T 1966-2007: Determination of chloramphenicol residues in aquatic product. Radio-receptor assay method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of chloramphenicol residues in aquatic product.Radio-receptor assay method Exit inspection and quarantine industry standard book People's Republic of China Detection of chloramphenicol residues in aquatic products Radioreceptor assay Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong CIQ. The main drafters of this standard. phase Dapeng Chen snow, Zhanglin Tian, Chen Yanqin, Liang Xiyang, Luo Huiming, £ - Zhuang Yilin. This standard is the first release of the entry-exit inspection and quarantine industry standards. Detection of chloramphenicol residues in aquatic products Radioreceptor assay1 ScopeThis standard specifies the determination of chloramphenicol residues in aquatic products for sample preparation and radioreceptor assay (CharmⅡ) method. This standard applies to the screening assays of chloramphenicol residues in aquatic products.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis3 Sample preparation and preservation3.1 Preparation of the sample All samples taken from a representative sample of not less than 500g, high-speed tissue masher mashing even the crushed sample is thoroughly mixed uniform. Loaded into a clean container, sealed and do identity. In the sample preparation process, should prevent sample contamination or the occurrence of the change of residue content. Samples for determining the number of bacteria can not exceed Over 106/g. 3.2 Sample preservation The sample was stored frozen at -18 ℃ below.4 Determination4.1 Principle Based assay is a competitive receptor immunoreactivity. After chloramphenicol residues in samples were extracted, and [3H] labeled chloramphenicol competition fine Chloramphenicol in bacterial cells specific receptors, chloramphenicol higher drug content in the sample, the more sites compete for binding, [3H] labeled chloramphenicol Biotin-binding sites of the less. After the reaction at a certain temperature, centrifuged, clear the [3H] labeled chloramphenicol unbound, scintillation fluid added after use LSC measurement sample of [3H] content cpm value (countperminute, that is, the number of pulses per minute). The lower the cpm value, The higher the sample chloramphenicol residues. 4.2 Reagents and materials 4.2.1 Water GB/T 6682 provided a water. The new kit batch performance monitoring shall be carried out before use. 1) CharmⅡ competitive receptor binding organization chloramphenicol test kit is provided by the trade name of the company's products Charm. This information is given The convenience of the user of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent product. 4.2.2.1 Competitive binding reagent. should be stored at -15 ℃ or less, up to 6h place at room temperature (15 ℃ ~ 25 ℃) under. 4.2.2.2 [3H] labeled chloramphenicol. should be stored at -15 ℃ or less, up to 6h place at room temperature (15 ℃ ~ 25 ℃) under. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1966-2007_English be delivered?Answer: Upon your order, we will start to translate SN/T 1966-2007_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1966-2007_English with my colleagues?Answer: Yes. 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