SN/T 1961.1-2007 English PDF
Basic dataStandard ID: SN/T 1961.1-2007 (SN/T1961.1-2007)Description (Translated English): Detection of allergen components in food. Part 1: Protocol of enzyme-linked immunosorbent assay (ELISA) for detecting peanut component Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.050 Word Count Estimation: 6,635 Date of Issue: 2007-08-06 Date of Implementation: 2008-03-01 Regulation (derived from): Accredidation-Technology-Letter [2015] No. 247 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the peanut allergens in food ingredients ELISA method. This standard applies to quantitative detection of cakes, candy, ice cream and other food allergens in peanut ingredients, other foods can refer to use. SN/T 1961.1-2007: Detection of allergen components in food. Part 1: Protocol of enzyme-linked immunosorbent assay (ELISA) for detecting peanut component---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detection of allergen components in food.Part 1. Protocol of enzyme-linked immunosorbent assay (ELISA) for detecting peanut component Exit inspection and quarantine industry standard book People's Republic of China Original component detection in food allergy Part 1. enzyme-linked immunosorbent assay peanut ingredients Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordSN/T 1961 "in food allergens detection method" is divided into two parts. --- Part 1. enzyme-linked immunosorbent assay peanut ingredients; --- Part 2. real-time PCR assay peanut ingredients. This section SN/T 1961 Part 1. This detection method is part of an international reference of Official Analytical Chemists Association (AOAC) and the US Food and Drug Administration (FDA) to make recommendation Correlation detection method Neogen company with production Veratox? Peanut allergen component quantitative test kit, verified by the test assessment Prepare proposed. Appendix A of this section is an informative annex. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section is drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters of this section. Luo Mao Huang, Zhang Xia, Zhang Haiying, Kit Cheng, Zhang Haibin, Gao Qi Li, Li diameter. The first part of the Department of Inspection and Quarantine issued by industry standards. Original component detection in food allergy Part 1. enzyme-linked immunosorbent assay peanut ingredients1 ScopeSN/T 1961 This section provides the ELISA method in food allergen peanut ingredients. This section applies to peanut allergens in food component quantitative detection cakes, candy, ice cream, etc., can refer to other food use.2 Detection2.1 Method summary Detection principle of this method is the sandwich ELISA method. The extracted sample of peanut protein bound to the antibody, was added Enzyme-labeled detection antibody bound with peanut protein binding, binding of the detection antibody reaction with the substrate color appears, read on a microplate reader Take absorbance with the absorbance of the standard controls of the standard curve, calculated by standard curve samples of peanut allergen content of the component. 2.2 equipment and appliances 2.2.1 Balance. Range 2kg, a sense of the amount of 0.1g. 2.2.2 homogenizer. 2.2.3 water bath, a hot plate or heat sources to maintain quite the 60 ℃ ± 1 ℃. 2.2.4 oscillator. 2.2.5 centrifuge. 2.2.6 50μL ~ 200μL adjustable pipette. 2.2.7 microplate reader. a wavelength of 650nm. 2.2.8 mortar and pulverization apparatus. 2.2.9 Reagent bottle. Capacity 1L. 2.2.10 peanut allergen component quantitative detection kit consisting See Appendix A. 2.2.11 washer. 2.3 Sample Preparation Sample taken 25g, with clean mortar or suitable grinding apparatus sample pulverized to a powder. 2.4 Sample extraction 1) Pretreatment 2.4.1 Extract. Take 1L10mmol/L phosphate buffer extract, stamped, shake mix, placed in a water bath so that the extract Preheated to 60 ℃. 1) This information is given for the convenience of users of the standard, it does not mean that the product approval procedures, as other products have different procedures, shall be tested After evaluating the use of verification. 2) This information is given for the convenience of users of the standard, it does not mean that the product approval procedures, as other products have different procedures, shall be tested After evaluating the use of verification. 2.4.2 Take 5g sample (liquid sample taken 5mL) added to the sample extraction cup for each sample at the same time to do two parallel samples. 2.4.3 Each sample cup was added 1.0g of peanut extract extracted additives, 125mL extract was preheated to 60 ℃, the cup tightly. 2.4.4 at 60 ℃ water bath to 150r/min shaking extraction cup 15min, so that part of the sample after standing 5min precipitation. 2.4.5 Take 5mL extract in 14000r/min centrifugal 5min (or low speed 2500r/min centrifugal 20min), the supernatant was put to the chamber Temperature can be used for testing. 2.5 Test Method 2) 2.5.1 Test shall all reagents to room temperature (18 ℃ ~ 30 ℃). ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1961.1-2007_English be delivered?Answer: Upon your order, we will start to translate SN/T 1961.1-2007_English as soon as possible, and keep you informed of the progress. 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