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SN/T 1942.2-2007 English PDF

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1942.2-2007399 Add to Cart 3 days Detection of Brucella in food from animal for import and export. Part 2: PCR method Obsolete

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Basic data

Standard ID: SN/T 1942.2-2007 (SN/T1942.2-2007)
Description (Translated English): Detection of Brucella in food from animal for import and export. Part 2: PCR method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050; 07.100.30
Word Count Estimation: 10,160
Date of Issue: 2007-08-06
Date of Implementation: 2008-03-01
Quoted Standard: GB 19489; SN/T 1942.1; WS/T 230-2002
Regulation (derived from): Accredidation-Technology-Letter [2015] No. 247
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of foodstuffs of animal origin Brucella PCR testing methods. This standard applies to import foodstuffs of animal origin Brucella rapid test.

SN/T 1942.2-2007: Detection of Brucella in food from animal for import and export. Part 2: PCR method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Brucella in food from animal for import and export.Part 2. PCR method Exit inspection and quarantine industry standard book People's Republic of China Import and export food of animal origin in Brucella Testing method Part 2. PCR testing methods Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1942-2007 "import and export food of animal origin in Brucella test method" is divided into two parts. --- Part 1. Separation and counting method; Part --- Article 2. PCR test. This section SN/T 1942 Part 2. This section of the Appendices A and B are normative appendix. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Liaoning Entry-Exit-Entry Inspection and Heilongjiang People's Republic of China Quarantine Bureau. The main drafters of this section. Woo, Moon Zheng, Jia , Lisu Long, LI Zhen-Rong, Hu Zhuanwei. This standard is the first release of the entry-exit inspection and quarantine industry standards. Import and export food of animal origin in Brucella Testing method Part 2. PCR testing methods

1 Scope

SN/T 1942 This section provides the import and export of foodstuffs of animal origin Brucella PCR test. This section applies to the import and export food of animal origin in rapid testing of the genus Brucella.

2 Normative references

The following documents contain provisions which, through reference SN/T 1942 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB 19489 General requirements for laboratory biosafety SN/T 1942.1 and export foodstuffs of animal origin Brucella test methods - Part 1. Separation and Counting Its application Technology WS/T 230-2002 clinical diagnosis Polymerase

3 Biosecurity

In order to protect the safety of laboratory personnel, the staff shall be qualified to detect Brucella, all cultures and waste should be careful Disposal. And in accordance with the relevant provisions of GB 19489. 4 anti-pollution measures Referring WS/T 230-2002 in Chapter 6, the prevention and control of pollution. 5 terms, definitions and abbreviations 5.1 Terms and Definitions The following terms and definitions apply to SN/T 1942 of this part. 5.1.1 Brucella is a class of Gram-negative bacilli short ball, biodegradable sugars such as glucose, less acid production, no power, no spores, peroxide Catalase positive. 5.1.2 Use two (usually a length of 15 to 25 nucleotides) as a reaction oligodeoxynucleotide primers, these two primer sequences should not Occurred complementary roles and specific sites can be tested on the two strands of DNA as a template occurs complementary respectively. The reaction solution containing magnesium include Reaction buffer ions, four kinds of deoxynucleotide triphosphates (of dNTP), a template DNA, primers and thermostable DNA polymerase composition. in DNA polymerase catalyzed by repeated changes of DNA denaturation, annealing and extension dozens of cycles obtained between two complementary sites Large number of copies of the DNA fragment.
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