SN/T 1777.1-2006 English PDF

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SN/T 1777.1-2006: Determination of macrolide residues in animal original food. Part 1: Radio-receptor assay method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1777.1-2006	449	Add to Cart	3 days	Determination of macrolide residues in animal original food. Part 1: Radio-receptor assay method	Valid

Similar standards

GB/T 9959.1   SB/T 10381   SB/T 10294   SN/T 5652.3   SN/T 4744   SN/T 1777.3   

Basic data

Standard ID: SN/T 1777.1-2006 (SN/T1777.1-2006)
Description (Translated English): Determination of macrolide residues in animal original food. Part 1: Radio-receptor assay method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X22;C53
Classification of International Standard: 67.120.01
Word Count Estimation: 14,182
Date of Issue: 2006-04-25
Date of Implementation: 2006-11-15
Quoted Standard: GB/T 6682
Regulation (derived from): Industry Standard Notice 2006 No. 7 (No. 79 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the organization of meat, offal and macrolide antibiotic residues in aquatic products Radioreceptor analysis (Charm ��) method. This standard applies to meat tissues, organs and macrolide antibiotic residues in aquatic products Determination.

SN/T 1777.1-2006: Determination of macrolide residues in animal original food. Part 1: Radio-receptor assay method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of macrolide residues in animal original food.Part 1. Radio-receptor assay method Book of the People's Republic of China Entry and Exit Inspection and Quarantine Macrolide antibiotic residues in animal-derived foods Methods of measurement - Part 1. Radioreceptor analysis Released on.2006-04-25 Implementation of.2006-11-15 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 1777 "Determination of macrolide antibiotic residues in animal-derived foods" is divided into two parts. --- Part 1. Radioreceptor analysis; --- Part 2. High performance liquid chromatography tandem mass spectrometry. This part is the first part of SN/T 1777. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Zhejiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Zhang Xiaofeng, Shi Weiliang, Su Zongren, Zhu Qingqing, Cheng Jie. This section is the first industry standard for entry-exit inspection and quarantine. Macrolide antibiotic residues in animal-derived foods Methods of measurement - Part 1. Radioreceptor analysis

1 Scope

This section of SN/T 1777 specifies radioreceptor analysis of macrolide antibiotic residues in meat tissues, viscera and aquatic products. (CharmII) method. This section applies to the determination of macrolide antibiotic residues in meat tissues, viscera and aquatic products.

2 Normative references

The terms in the following documents become the provisions of this section by reference to this part of SN/T 1777. All dated references The document, all subsequent amendments (not including the contents of the errata) or revisions do not apply to this section, however, encouragement is based on this section. The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to This section. GB/T 6682 Analytical laboratory water specifications and test methods

3 Preparation and preservation of samples

3.1 Preparation of samples A representative sample is taken from all the samples, and a sample of not less than 1000 g is shrunk by a quarter method, and the fat is removed, thoroughly mixed, and mixed. Divide into two parts, put in a clean container, seal and mark. Fat is an interfering substance and should be removed as much as possible. the preparation process should ensure that the meat is not contaminated by microorganisms before extraction. 3.2 Storage of samples Store samples at -20 ° C ~ -18 ° C, fresh or frozen tissue samples (except viscera) can be stored at 2 ° C ~ 6 ° C 72h.

4 Determination method

4.1 Principle The Radiation Receptor Analysis (CharmII) method is based on the binding of drug functional groups to microbial receptor sites. A common functional group of a class of antibiotics is associated. This specificity of the application of the receptor allows for multi-residue analysis of a certain class of antibiotics. This party In the method, the macrolide residues remaining in the sample are extracted and diluted to compete with the [14C]-labeled erythromycin for binding to specific receptor sites. point. After a certain temperature reaction, centrifuge to separate unbound macrolides, and finally add scintillation to determine [14C] decay. The beta particle radioactivity is counted as cpm (countperminute). The measured cpm value is determined by the amount of macrolide antibiotic residues in the sample. Inverse ratio. 4.2 Reagents and materials Unless otherwise stated, the reagents used are of excellent grade and the water is the first grade water specified in GB/T 6682. 4.2.1 Macrolide test kit. a) Tissue negative control concentrated dry powder. dry powder is stored at 2 ° C ~ 6 ° C, when used, concentrated dry powder is labeled as negative on the label The prepared solution can be stored in a refrigerator or ice bath at 2 ° C ~ 6 ° C for 48 h. If not used for a long time or estimated within 2d After use, all or part of the solution can be stored in a refrigerator below -15 ° C, and thawed when used. The thawed solution is in ......

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