SN/T 1772-2006 English PDF
Basic dataStandard ID: SN/T 1772-2006 (SN/T1772-2006)Description (Translated English): Determination of zearalenone in cereals for import and export. Immunoaffinity column and liquid chromatoraphy method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B20;X11 Classification of International Standard: 67.060 Word Count Estimation: 13,133 Date of Issue: 2006-04-25 Date of Implementation: 2006-11-15 Regulation (derived from): Industry Standard Notice 2006 No. 7 (No. 79 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the import and export of zearalenone in cereals test sample preparation and immunoaffinity column liquid chromatographic method. This standard applies to import corn, wheat zearalenone test. SN/T 1772-2006: Determination of zearalenone in cereals for import and export. Immunoaffinity column and liquid chromatoraphy method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of zearalenone in cereals for import and export.Immunoaffinity column and liquid chromatoraphy method Book of the People's Republic of China Entry and Exit Inspection and Quarantine Determination of Zearalenone in Import and Export Grains Immunoaffinity column-liquid chromatography Released on.2006-04-25 Implementation of.2006-11-15 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordAppendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Yan Kai, Li Jun, Wei Feng, Song Wenbin, Lin Weixuan, Tian Miao. This standard is the first industry standard for entry-exit inspection and quarantine. Determination of Zearalenone in Import and Export Grains Immunoaffinity column-liquid chromatography1 ScopeThis standard specifies the preparation of zearalenone in the import and export grain and the method of immunoaffinity column-liquid chromatography. This standard is applicable to the inspection of corn zearalenone in corn and wheat.2 sample preparation2.1 Sample preparation The sample was reduced to 1 kg by a quarter method, all of which was ground and passed through a 20-mesh sieve, mixed, and divided into two portions as a sample. Separately loaded Inside the sample container, sealed and marked. During the sampling and sample preparation operations, the sample should be protected from contamination or the content of the sample to be tested. The change. 2.2 Sample storage The sample was stored at -5 ° C or less in the dark.3 Determination method3.1 Method summary After the zearalenone in the sample was extracted with acetonitrile-water, the extract was purified by an immunoaffinity column. Using liquid color with a fluorescence detector The spectrometer was measured and quantified by external standard method. 3.2 Reagents and materials Unless otherwise specified, the reagents used were of analytical grade and the water was distilled water or equivalent deionized water. 3.2.1 Methanol. HPLC grade. 3.2.2 Acetonitrile. HPLC grade. 3.2.3 Acetonitrile + water (9+1). Take 90 mL of acetonitrile plus 10 mL of water. 3.2.4 Sodium chloride. 3.2.5 Zearalenone standard. purity ≥ 98%. 3.2.6 Zearalenone standard solution. accurately weigh the appropriate amount of zearalenone standard, and the concentration of acetonitrile is 0.1mg/mL. Standard stock solution. Dilute to the appropriate concentration of standard working solution with mobile phase as needed. 3.3 Instruments and equipment 3.3.1 The liquid chromatograph is equipped with a fluorescence detector. 3.3.2 Crusher. 3.3.3 High speed homogenizer. 3.3.4 Nitrogen blowing instrument. 3.3.5 Air pressure pump. 3.3.6 Glass fiber filter paper. 3.3.7 Glass syringe. 20mL. 3.3.8 Immunoaffinity column. Zearalenone immunoaffinity column. 3.3.9 Microinjector. 100 μL. 3.4 Determination steps 3.4.1 Extraction Weigh about 40g (accurate to 0.1g) in a 250mL stoppered conical flask, add 4g sodium chloride and 100mL acetonitrile + water ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1772-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1772-2006_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1772-2006_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1772-2006_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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