SN/T 1771-2006 English PDF
Basic dataStandard ID: SN/T 1771-2006 (SN/T1771-2006)Description (Translated English): Determination of T-2 toxin in cereals for import and export - Immunoaffinity column and liquid chromatography method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B20;X11 Classification of International Standard: 67.060 Word Count Estimation: 12,192 Date of Issue: 2006-04-25 Date of Implementation: 2006-11-15 Regulation (derived from): Industry Standard Notice 2006 No. 7 (No. 79 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the import and export of T-2 toxin in cereals test sample preparation and immunoaffinity column liquid chromatographic method. This standard applies to import corn, wheat T-2 toxin test. SN/T 1771-2006: Determination of T-2 toxin in cereals for import and export - Immunoaffinity column and liquid chromatography method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of T-2 toxin in cereals for import and export-Immunoaffinity column and liquid chromatography method Book of the People's Republic of China Entry and Exit Inspection and Quarantine Determination of T-2 toxin in import and export grain Immunoaffinity column-liquid chromatography Released on.2006-04-25 Implementation of.2006-11-15 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordAppendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard is undertaken by the Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and the Shanghai Entry-Exit Inspection and Quarantine Bureau Drafting. The main drafters of this standard. Wei Feng, Li Jun, Yan Kai, Fang Xiaoming. This standard is the first industry standard for entry-exit inspection and quarantine. Determination of T-2 toxin in import and export grain Immunoaffinity column-liquid chromatography1 ScopeThis standard specifies the preparation of T-2 toxin test in the import and export grain and the method of immunoaffinity column-liquid chromatography. This standard applies to the import and export of corn and wheat T-2 toxin test.2 sample preparation2.1 Sample preparation The sample was reduced to 1 kg by quartering, all ground and passed through a 20 mesh sieve, mixed, and divided into two portions as samples, respectively Inside the container, seal and mark it. Samples should be protected from contamination or residue levels during sampling and sample preparation operations. Variety. 2.2 Sample storage The sample was stored at -5 ° C or less in the dark.3 Determination method3.1 Method summary After the T-2 toxin in the sample is extracted with methanol-water, the extract is purified by immunoaffinity column, concentrated, derivatized, and adjusted to volume. The liquid chromatograph of the detector was measured and quantified by an external standard method. 3.2 Reagents and materials Unless otherwise specified, the reagents used were of analytical grade and the water was double distilled water. 3.2.1 Methanol. 3.2.2 Acetonitrile. HPLC grade. 3.2.3 Toluene. HPLC grade. 3.2.4 Methanol + water (8 + 2). Take 80 mL of methanol plus 20 mL of water. 3.2.5 0.325mg/mL 4-dimethylaminopyridine (DMAP) solution. Accurately weigh 0.0325g in a 100mL volumetric flask, toluene Released to the scale. 3.2.6 0.3mg/mL1-anthroylnitrile (1-AN) solution. accurately weigh 0.0300g in a 100mL volumetric flask, A Dilute benzene to the mark. 3.2.7 T-2 toxin standard. purity ≥ 98%. 3.2.8 T-2 toxin standard solution. accurately weigh the appropriate amount of T-2 toxin standard, and prepare a standard storage of 0.5mg/mL with acetonitrile. Preparation. Dilute at the required concentration and use as a standard working solution. 3.3 Instruments and equipment 3.3.1 The liquid chromatograph is equipped with a fluorescence detector. 3.3.2 Crusher. 3.3.3 High speed homogenizer. 3.3.4 Nitrogen blowing instrument. 3.3.5 Air pressure pump. 3.3.6 Glass fiber filter paper. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1771-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1771-2006_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1771-2006_English with my colleagues?Answer: Yes. 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