SN/T 1697-2006 English PDF
Basic dataStandard ID: SN/T 1697-2006 (SN/T1697-2006)Description (Translated English): Protocol of blocking ELISA for differentiating antibodies of swine transmissible gastroenteritis virus and porcine respiratory coronavirus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 6,675 Date of Issue: 1/26/2006 Date of Implementation: 2006-08-16 Quoted Standard: GB/T 6682 Regulation (derived from): ?Industry Standard Announcement 2006 No.4 (Total No.76); National-Quality-Inspection-accreditation (2010) 290; Industry Standard Filing Announcement 2010 No.10 (Total No.130) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the porcine transmissible gastroenteritis (TGE) virus blocking ELISA antibody identification test operations technical requirements. This standard applies to porcine transmissible gastroenteritis virus, porcine respiratory coronavirus identified. SN/T 1697-2006: Protocol of blocking ELISA for differentiating antibodies of swine transmissible gastroenteritis virus and porcine respiratory coronavirus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Protocol of blocking ELISA for differentiating antibodies of swine transmissible gastroenteritis virus and porcine respiratory coronavirus People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Porcine transmissible gastroenteritis virus and porcine respiratory coronavirus Antibody Blocking ELISA Identification Test Procedure Released on.2006-01-26 Implementation of.2006-08-16 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordAppendix A, Appendix B and Appendix C of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Luo Changbao, Chen Ru, Liu Linlin. This standard is the first industry standard for entry-exit inspection and quarantine. Porcine transmissible gastroenteritis virus and porcine respiratory coronavirus Antibody Blocking ELISA Identification Test Procedure1 ScopeThis standard specifies the ELISA test for porcine transmissible gastroenteritis (TGE) virus and porcine respiratory coronary (PRC) virus antibody blocking ELISA. Operational technical requirements. This standard applies to the identification of porcine transmissible gastroenteritis virus and porcine respiratory coronavirus.2 Normative referencesThe terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and experimental methods3 materials3.1 Viruses and cells ST cells were grown in 96-well cell culture plates to form a monolayer and infected with the TGE virus Miller6 strain. Cytopathic rate after 24h Fix cells at no more than 10%. 3.2 Fixed cells The cell plate was washed once with PBS (pH 7.4) for 1 to 2 times, then fixed with 80% acetone (precooled -20 ° C) for 15 min, and the acetone was discarded. Dry in a wind cabinet for 10 min, then store at -20 °C for later use. 3.3 antibody Biotinylated anti-TGE virus, A site monoclonal antibody, anti-TGE virus D site monoclonal antibody.4 method of operation4.1 Remove the plate from -20 °C and set at room temperature. After blocking the non-specific site for 2 h at room temperature (about 25 °C) with PBST (see Appendix A), remove it. PBST was patted dry on a paper towel and 150 μL of PBST was added to all virus-infected wells. 4.2 Add 50 μL of positive serum to A1 and A2, negative serum B1 and B2 (ie, double well), and other tested serums are listed in Appendix B. 4.3 Dilute the sample cell plate at 4 ° C overnight. 4.4 Drain the diluted sample solution and do not rinse. 4.5 Add 100 μL of working concentration biotinylated TGEA site monoclonal antibody to each well. 4.6 For identification purposes, each sample is diluted in two plates, each plate should be operated separately, using different monoclonal antibodies, separate Count, and contain their respective negative and positive serum. 4.7 At room temperature for 2 h or 4 ° C overnight. 4.8 Wash three times with PBST. 4.9 Add 100 μL of working concentration horseradish peroxidase-labeled avidin to each well and incubate for 30 min to 40 min at room temperature. 4.10 Wash PBST three times. 4.11 Add 100μLABTS to each well (see Appendix C). 4.12 After 30 min at room temperature, 100 μL of sodium 5% sodium dodecyl sulfate (mass concentration) was added to stop the reaction. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1697-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1697-2006_English as soon as possible, and keep you informed of the progress. 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