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SN/T 1699-2017 English PDF

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Q: Should I purchase the latest version SN/T 1699-2017?

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US$399.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1699-2017: Quarantine protocol for pofcine epidemic diarrhea
Status: Valid

SN/T 1699: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1699-2017	399	Add to Cart	4 days	Quarantine protocol for pofcine epidemic diarrhea	Valid
SN/T 1699.1-2006	239	Add to Cart	3 days	Protocol of micro-serum neutralization test for porcine epidemic diarrhea	Obsolete

Similar standards

GB/T 20365 SN/T 1353 NY/T 909 SN/T 5671 SN/T 1694 SN/T 1698

Basic data

Standard ID: SN/T 1699-2017 (SN/T1699-2017)
Description (Translated English): Quarantine protocol for pofcine epidemic diarrhea
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 17,134
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Older Standard (superseded by this standard): SN/T 1699.2-2006
Quoted Standard: GB/T 6682
Regulation (derived from): National Quality Inspection (2017) No. 337
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the isolation and identification of porcine epidemic diarrhea, reverse transcription polymerase chain reaction, real-time fluorescent reverse transcription polymerase chain reaction, direct immunofluorescence assay, enzyme-linked immunosorbent assay and micro-sera neutralization assay. Laboratory quarantine technical specifications. This standard is applicable to the quarantine, epidemic monitoring and diagnosis of epidemic diarrhea in pigs entering and leaving the country.

SN/T 1699-2017: Quarantine protocol for pofcine epidemic diarrhea

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for pofcine epidemic diarrhea People's Republic of China entry and exit inspection and quarantine industry standards Replace SN/T 1699.1-2006, SN/T 1699.2-2006, SN/T 1699.3-2006 Technical specification for quarantine of swine epidemic diarrhea Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1699.1-2006 "Microseline Neutralization Test Procedures for Porcine Epidemic Diarrhea", SN/T 1699.2-2006 "Directive immunofluorescence test protocol for swine epidemic diarrhea virus", SN/T 1699.3-2006 "Indirect spot enzyme coupling for pig epidemic diarrhea" Operational Procedures for Epidemic Adsorption Tests. Main technical changes compared with SN/T 1699.1-2006, SN/T 1699.2-2006, SN/T 1699.3-2006 as follows. --- Increased the method for isolation and identification of porcine epidemic diarrhea virus; --- Added reverse transcription-polymerase chain reaction method; --- Added real-time fluorescence reverse transcription polymerase chain reaction method; --- Increased the enzyme-linked immunosorbent assay method, replacing the indirect dot enzyme-linked immunosorbent assay protocol; --- Revised the direct immunofluorescence test protocol, adopting NY/T 544-2015 "Diagnostic Techniques for Porcine Epidemic Diarrhea" 6.2 “Direct immunofluorescence method” and detailed description of its operation steps; --- Revised the micro-sera neutralization test protocol, recommended cell lines, virus proliferation and toxic rate determination, control settings, etc. The content has been revised and supplemented. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, South China Agricultural University, Shenzhen, People's Republic of China Entry Inspection and Quarantine Bureau. The main drafters of this standard. Chen Ru, Ma Baohua, Ma Jingyun, Ai Qing, Zhu Daozhong, Cheng Yiyi, Qin Zhifeng, Yang Junxing, Yang Jian, Liu Zhiling, Wu Yiwen, Luo Qiong, Duan Yanyu, Sun Jie, Lin Zhixiong, Chen Bing, Wang Ying, Yu Haiqiong. The previous versions of the standards replaced by this standard are. ---SN/T 1699.1-2006; ---SN/T 1699.2-2006; ---SN/T 1699.3-2006. Technical specification for quarantine of swine epidemic diarrhea

1 Scope

This standard specifies the isolation and identification of porcine epidemic diarrhea, reverse transcription polymerase chain reaction, real-time fluorescent reverse transcription polymerase chain Laboratory quarantine specifications for reactions, direct immunofluorescence assays, enzyme-linked immunosorbent assays, and microserum neutralization assays. This standard is applicable to the quarantine, epidemic monitoring and diagnosis of epidemic diarrhea in pigs entering and leaving the country.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. PED. Porcine epidemic diarrhea (porcineepidemicdiarrhea) PEDV. Porcine epidemic diarrhea virus (porcineepidemicdiarrheavirus) PBS. phosphate buffer (phosphatebuffersaline) Vero. African Green Monkey Kidney Cells DMEM. an improved cell culture fluid (dulbecco'smodifiedeaglemedium) CPE. cytopathic effect RT-PCR. reverse transcription-polymerase chain reaction (reversetranscription-polymerase chain reaction) ddH2O. double distilled water (doubledistiledwater) DEPC. diethylpyrocarbonate BSA. bovine serum albumin (bovineserumalbumin) Ct. number of cycles experienced when the fluorescence signal reaches the set threshold FITC. fluorescein isothiocyanate ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay) TMB. 3,3',5,5'-tetramethylbenzidine (3,3',5,5'-Tetramethylbenzidine) OD. optical density (optical density) TCID50. half tissue culture infection dose (tissuecultureinfectivedose)

4 Overview of porcine epidemic diarrhea

Porcine epidemic diarrhea (PED) is a porcine epidemic diarrhea virus (PEDV) from Coronaviridae, Coronavirus An acute highly exposed swine intestinal infectious disease with clinical manifestations of watery diarrhea, vomiting, dehydration, exercise stiffness and high death in suckling piglets Characteristics such as death rate. Pigs of all ages can be infected, with lactating piglets at 1 week of age being the most susceptible. PEDV mainly through infected pigs The spread of feces or other viral contaminants, under natural conditions, PEDV enters the pig's body through the oral and nasal routes. The prevalence of PED ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1699-2017_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1699-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1699-2017_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1699-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version SN/T 1699-2017?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1699-2017 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.