SN/T 1695-2006 English PDF
Basic dataStandard ID: SN/T 1695-2006 (SN/T1695-2006)Description (Translated English): Horse piroplasmosis micro complement fixation test operating procedures Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 10,122 Date of Issue: 2006-01-26 Date of Implementation: 2006-08-16 Quoted Standard: GB/T 6682 Regulation (derived from): Industry Standard Notice 2006 No. 4 (total of 76) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the horse piroplasmosis micro complement fixation test method of operation. This standard applies to the horse equine piroplasmosis serological diagnosis. SN/T 1695-2006: Horse piroplasmosis micro complement fixation test operating procedures---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Horse piroplasmosis micro complement fixation test operating procedures Book of the People's Republic of China Entry and Exit Inspection and Quarantine Majiaosis micro-complement binding test operation procedure Released on.2006-01-26 Implementation of.2006-08-16 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard is mainly based on the OIE "Diagnostic Reagents and Vaccine Standards Manual" (2004) fifth edition, Chapter 2.5.6. Appendix A, Appendix B and Appendix C of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Jian Zhongyou, Fu Lei, Hu Chuanwei, Sun Yanfeng, Su Yongsheng, Sun Yingjie. This standard is the first industry standard for entry-exit inspection and quarantine. Majiaosis micro-complement binding test operation procedure1 ScopeThis standard specifies the method of operation for the micro-complement binding test of horse coccidiosis. This standard applies to the serological diagnosis of equine animal horse coccidiosis.2 Normative referencesThe terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods3 reagents and materials3.1 Reagents 3.1.1 The distilled water used for the test is the tertiary water in GB/T 6682. 3.1.2 Diluent. Barbiturate buffer (see Chapter A.2). 3.1.3 Complement-bound antigen. provided by the designated unit, used according to the instructions. 3.14 Standard positive serum. provided by the designated unit, used according to the instructions. 3.1.5 Standard negative serum. provided by the designated unit, used according to the instructions. 3.1.6 Hemolysin. Freeze-dried product, dissolved in distilled water to the specified volume before use. 3.1.7 Complement. Freeze-dried product, dissolved in distilled water to the specified volume before use. 3.1.8 sheep red blood cell storage solution. choose healthy adult sheep, blood sampling in sterile jugular vein, 1.2 parts of aristoleine (see chapter A.1) plus 1 part of blood Liquid, mix, store at 2 ° C ~ 8 ° C, use within 2 weeks. 3.2 Tested serum 3.2.1 Blood collection and separation of serum Venous blood was collected according to a conventional method, and serum was aseptically separated. Serum must be fresh, free of protein flocs, no hemolysis, no spoilage. 3.2.2 Shipping and preservation of serum Serum is shipped refrigerated. If it cannot be sent to the laboratory within 3 days, it should be shipped by freezing. 3.3 Materials 3.3.1 Glass test tube. 10mm × 75mm, round bottom clean reaction tube. 3.3.2 Test tube rack. 3.3.3 Micropipettes. 3.3.4 Adjustable continuous sampler. 3.3.5 96-well "U" type microplate.4 method of operation4.1 preliminary test 4.1.1 Preparation of 3% red blood cell suspension 4.1.1.1 After the sheep's whole blood is thoroughly mixed, take the required amount and wash it three times with the dilution solution, centrifuge at.2000r/min for 5min each time, and last time away. Heart 10min. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1695-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1695-2006_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). 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