SN/T 1692.1-2006 English PDF
Basic dataStandard ID: SN/T 1692.1-2006 (SN/T1692.1-2006)Description (Translated English): Protocol of agar-gel immunodiffusion test for African horse sickness Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 5,586 Date of Issue: 2006-01-26 Date of Implementation: 2006-08-16 Quoted Standard: GB/T 6682 Regulation (derived from): Industry Standard Notice 2006 No. 4 (total of 76) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the African horse sickness agar immunodiffusion test procedures. This standard applies to African horse sickness AGP antibodies. SN/T 1692.1-2006: Protocol of agar-gel immunodiffusion test for African horse sickness---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Protocol of agar-gel immunodiffusion test for African horse sickness Book of the People's Republic of China Entry and Exit Inspection and Quarantine African horse stable agar immunodiffusion test operation procedure Released on.2006-01-26 Implementation of.2006-08-16 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis section is based on the World Organisation for Animal Health (OIE) Handbook of Diagnosis and Vaccines for Terrestrial Animal Diseases. SN/T 1692 is divided into three parts. --- African horse stable agar immunodiffusion test operating procedures; --- African horse stable hemagglutination and hemagglutination inhibition test procedures; --- African horse stable complement test procedures. This part is the first part of SN/T 1692. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Yang Jingyan, Xu Zizhong, Chen Jianjun, Long Zhongbao. This section is the first industry standard for entry-exit inspection and quarantine. African horse stable agar immunodiffusion test operation procedure1 ScopeThis part of SN/T 1692 specifies the protocol for the African equine agar immunodiffusion test. This section applies to the detection of African horseshoe agaric expansion antibodies.2 Normative referencesThe terms in the following documents become the provisions of this part by reference to this part of SN/T 1692. Quotations with dated , all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this section. GB/T 6682 Analytical laboratory water specifications and test methods3 reagents and materials3.1 Antigen. African horse stable tissue culture antigen. 3.2 African horse stable positive serum. 3.3 plate. 3.4 Micropipettes. 3.5 micro dripper. 3.6 Erlenmeyer flask. 3.7 agarose. 3.8 Sodium chloride. analytically pure. 3.9 Sodium azide. analytically pure. 3.10 Water. Meet the secondary water specifications of the GB/T 6682 analytical laboratory.4 steps4.1 Preparation of agar gel plate Weigh 1.2g of agarose, 1.0g of sodium chloride, add 100mL of water, dissolve it by high pressure or boiling after 68.94kPa, 10min, then cool to At 45 ° C, 0.01 g of sodium azide was added. Add agar to each plate and make the agar plate thickness 2.5mm~2.8mm, and then invert after solidification. Put in a refrigerator at 4 ° C for 15 min. 4.2 punching 1 hole in the middle and 6 holes in the surrounding group. The aperture is 4 mm and the hole spacing is 2.4 mm. Use a needle to pick out or aspirate the agar in the well. 4.3 Loading The central well was added with antigen, 1, 3, and 5 wells plus standard positive serum; 2, 4, and 6 wells were added to the test serum. The amount of sample is based on the hole level, and the sample is placed after the wet box. The reaction was carried out at room temperature (22 ° C to 25 ° C), and a positive control was set for each test.5 judgment5.1 24h initial judgment, 72h final judgment. When the positive control is established, a determination is made. Positive. a dense sediment line appears between the test serum well and the antigen well, and is smooth with the end of the sedimentation line of the standard positive serum. Connected. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1692.1-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1692.1-2006_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1692.1-2006_English with my colleagues?Answer: Yes. 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