SN/T 1681-2023 English PDFSN/T 1681: Historical versions
Basic dataStandard ID: SN/T 1681-2023 (SN/T1681-2023)Description (Translated English): (Technical specifications for quarantine of honey bee larval putrid disease) Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 65.020.30 Date of Issue: 2023-11-01 Date of Implementation: 2024-05-01 Older Standard (superseded by this standard): SN/T 1681-2011 Issuing agency(ies): General Administration of Customs SN/T 1681-2011: Quarantine protocol for American foulbrood of honey bees---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Quarantine protocol for American foulbrood of honey bees People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1681-2005 American bee larvae rancid disease quarantine Technical Specifications Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces the SN/T 1681-2005 "bee larvae rancid American disease diagnosis." This standard compared with SN/T 1681-2005, the main technical changes are as follows. --- Changing the name of the standard; --- Increased "site inspection and quarantine," "physiological characterization" and "PCR Detection" content; --- Modification of the original standard "sampling", "direct test" and "Isolation and identification." This revised standard adopts Office International des Epizooties (OIE) "Terrestrial animal disease diagnostic tests and vaccines Standards Manual" (2008 edition) 2.2.2 Chapter, the main change is as follows. --- Description of "introduction" and so, as amended, abbreviated as the present standard in Appendix A; --- In its original format and content have been adjusted on the order of typesetting. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine. The main drafters of this standard. Guo STARS Zengxian Dong, Pinghan Li, Chen Jianjun, Zhao Hui, Yan Zhili, Zhu Tong Ming Wang Zhenhua. This standard replaces the standards previously issued as follows. --- SN/T 1681-2005. American bee larvae rancid disease quarantine Technical Specifications1 ScopeThis standard specifies the Americas bee larvae rancid disease site inspection and quarantine, sampling, laboratory diagnosis and quarantine technical specifications. This standard applies to the entry and exit of American bee larvae rancid disease quarantine and epidemiology, diagnosis and monitoring of the disease.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 18088 Entry and Exit Animal Quarantine sampling3 Reagents and materials3.1 Bacillus larvae (Paenibacilluslarvaesubsp.larvae) Standard strains designated by the OIE Reference Laboratory or the State Administration of Quality Supervision, Inspection and Quarantine laboratory. 3.2 Primer 3.2.1 PCR primers Upstream primer. 5'-CTTGTGTTTCTTTCGGGAGACGCC-3 ' Downstream primer. 5'-TCTTAGAGTGCCCACCTCTGCG-3 ' Amplified fragment size. 1106bp. 3.2.2 Nested PCR primers 3.2.2.1 outer nested PCR primers Upstream primer. 5'-TCGAGCGGACCTTGTGTT-3 ' Downstream primer. 5'-CTATCTCAAAACCGGTCAGAG-3 ' Amplified fragment size. 969bp. 3.2.2.2 medial nested PCR primers Upstream primer. 5'-CTTCGCATGAAGTCATG-3 ' Downstream primer. 5'-TCAGTTATAGGCCAGAAAGC-3 ' Amplified fragment size. 572bp. 3.3 Taq enzyme. 3.4 dNTPs. containing dCTP, dGTP, dATP, dTTP each 10mmol/L. 3.5 DNAMarker. 3.6 ethidium bromide. 3.7 isopropanol. 3.8 10mmol/LTris ยท HCl-1mmol/LEDTA solution (pH8.0). 3.9 carrot yeast agar medium. 3.10 Potato media. 3.11 yeast extract media. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1681-2023_English be delivered?Answer: Upon your order, we will start to translate SN/T 1681-2023_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version SN/T 1681-2023?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1681-2023 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
