SN/T 1612-2013 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1612-2013	639	Add to Cart	3 days	Detection and identification of Carnation ringspot virus	Obsolete
SN/T 1612-2005	239	Add to Cart	2 days	Carnation ringspot virus serological detection methods	Obsolete

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Basic data

Standard ID: SN/T 1612-2013 (SN/T1612-2013)
Description (Translated English): Detection and identification of Carnation ringspot virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020
Word Count Estimation: 16,124
Older Standard (superseded by this standard): SN/T 1612-2005
Quoted Standard: SN/T 1840
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method of entry quarantine identified seedlings in Carnation ringspot virus DAS-ELISA, immuno-electron microscopy, immuno-capture and real-time RT-PCR and RT-PCR. This standard applies to the quarantine identify seedlings in Ca

SN/T 1612-2013: Detection and identification of Carnation ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Carnation ringspot virus People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1612-2005 Carnation ringspot virus quarantine and identification methods Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1612-2005 "Carnation ringspot virus serological detection methods." This standard compared with SN/T 1612-2005, the main technical changes are as follows. --- Increased immuno-capture by RT-PCR method; --- Increased immune electron microscopy method; --- Adds real-time fluorescent RT-PCR detection method; --- Added Appendix Carnation ringspot virus related information. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard by the People's Republic of China Shanghai Exit Inspection and Quarantine is responsible for drafting. The main drafters of this standard. Yang Cuiyun at Chui, Hupei Long, Tao Tingdian, Cuixue Hui, in the sub-Xiang. This standard replaces the standards previously issued as follows. --- SN/T 1612-2005. Carnation ringspot virus quarantine and identification methods

1 Scope

This standard specifies the entry seedlings in Carnation ringspot virus DAS-ELISA, immuno-electron microscopy, immuno-capture RT-PCR and real Quarantine and identification of fluorescent RT-PCR and the like. This standard applies to the quarantine seedlings in the identification of Carnation ringspot virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method

3 Carnation ringspot virus basic information

Chinese name. Carnation ringspot virus English name. Carnationringspotvirus Abbreviation. CRSV Taxonomic position. tomato bushy stunt virus family (Tombusviridae), carnation ringspot virus genus (Dianthovirus) Transmission. The virus spread through long distance transport of vegetative propagation material, mainly due to the close contacts between plants, soil And soil pollution caused by farming operations spread of the virus. For additional information, see Appendix Carnation ringspot virus A.

4 principle of the method

Carnation ringspot virus has a moderate to strong immunogenicity, easy to obtain high-quality multi antiserum weaker between serum within the genus virus Cross-reactive. In Carnation ringspot virus antiserum, using DAS-ELISA or immuno-capture RT-PCR screening for viruses, if the sample is positive Resistance, the real-time fluorescent RT-PCR, immune electron microscopy, or differential host reactions, positive material verification. And two kinds of the above methods Identification of the type of virus quarantine. 5 equipment, appliances and reagents 5.1 Equipment Microplate reader, PCR, real-time PCR instrument, clean benches, electronic scales (1/10000g), electrophoresis, electrophoresis tank, gel imaging System, water bath, high-speed refrigerated centrifuge, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave, Vortex. 5.2 Appliances Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no ......

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