SN/T 1617-2017 English PDF

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SN/T 1617-2017: Detection and identification of Cacao swollen shoot virus
Status: Valid

SN/T 1617: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1617-2017	349	Add to Cart	3 days	Detection and identification of Cacao swollen shoot virus	Valid
SN/T 1617-2005	439	Add to Cart	3 days	Detection of Cacao swollen shoot virus	Obsolete

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NY/T 2288   SN/T 1204   JB 2293   SN/T 1619   SN/T 1618   SN/T 1616   

Basic data

Standard ID: SN/T 1617-2017 (SN/T1617-2017)
Description (Translated English): Detection and identification of Cacao swollen shoot virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020
Word Count Estimation: 15,155
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Older Standard (superseded by this standard): SN/T 1617-2005
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the detection method of cocoa swollen virus. This standard applies to the quarantine test of cocoa swollen virus in imported plant materials.

SN/T 1617-2017: Detection and identification of Cacao swollen shoot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cacao swollen shoot virus People's Republic of China entry and exit inspection and quarantine industry standards Replace SN/T 1617-2005 Cocoa swollen virus quarantine and identification method Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1617-2005 "Detection method of cocoa swollen virus", compared with SN/T 1617-2005, the main technical changes The following is as follows. --- Improve the original standard detection method and increase the real-time fluorescent PCR detection method; --- Improved the original standard genomic content; --- Removed the immuno-trap PCR method from the original standard and added the PCR method; --- Revised the judgment of the results in the original standard; --- Modified the appendix of the original standard. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. China Academy of Inspection and Quarantine, Zhongshan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese People And the Jiangsu Entry-Exit Inspection and Quarantine Bureau, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wei Meisheng, Chen Dinghu, Xiang Ning, Li Guifen, Wu Cuiping, Guo Jingze. The previous versions of the standards replaced by this standard are. ---SN/T 1617-2005. Cocoa swollen virus quarantine and identification method

1 Scope

This standard specifies the detection method of cocoa swollen virus. This standard applies to the quarantine test of cocoa swollen virus in imported plant materials.

2 basic information of cocoa swollen virus

Scientific name. Cacaoswollenshootvirus Abbreviation. CSSV Chinese name. Cocoa swollen virus Synonym. Theobromavirus1; Marmortheobromae; Theobromavirusinflans. Classification status. Caulimoviridae; Badnavirus. See Appendix A for additional information on CSSV.

3 Principle of the method

The protein and genomic characteristics of CSSV are the basis for the quarantine and identification of this virus. According to the specific reaction between CSSV and antibody, Plant samples were subjected to double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); PCR was performed based on the genomic characteristics of CSSV, real-time Fluorescent PCR; by an effective combination of these methods, determine if the sample carries CSSV. 4 instruments, appliances and reagents 4.1 Instrumentation Enzyme-linked detector, balance (sensitivity 0.001g), PCR instrument, electrophoresis instrument, real-time fluorescence PCR instrument, electrophoresis tank, UV transilluminator, isolation temperature Room, juicer, water bath, etc. 4.2 Appliances Adjustable pipettes (200μL, 20μL, 2μL), adjustable pipette tips, enzyme plates, centrifuge tubes, finger tubes, mortars, etc. 4.3 Reagents Enzyme-linked assay reagents (see Appendix B) and polymerase chain reaction assay reagents (see Appendix C).

5 Detection methods

5.1 Double antibody sandwich enzyme-linked immunosorbent assay See Appendix B. ......

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