SN/T 1611-2013 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1611-2013	479	Add to Cart	3 days	Serological detection methods of Southern bean mosaic virus	Obsolete
SN/T 1611-2005	279	Add to Cart	3 days	Southern bean mosaic virus serological detection methods	Obsolete

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Basic data

Standard ID: SN/T 1611-2013 (SN/T1611-2013)
Description (Translated English): Serological detection methods of Southern bean mosaic virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B23
Classification of International Standard: 67.060
Word Count Estimation: 12,130
Older Standard (superseded by this standard): SN/T 1611-2005
Quoted Standard: SN/T 2122; SN/T 1840
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the plant quarantine in Southern bean mosaic virus detection method based DAS- ELISA serology, immune electron microscopy and immuno-capture and RT-PCR. This standard applies to detect the entry and exit legume seeds, seedlings and

SN/T 1611-2013: Serological detection methods of Southern bean mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Serological detection methods of Southern bean mosaic virus People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1611-2005 Southern bean mosaic virus serological methods Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1611-2005 "Southern bean mosaic virus serological detection methods." This standard compared with SN/T 1611-2005, the main technical changes are as follows. --- Increased immune electron microscopy method; --- Increased immuno-capture RT-PCR validation method; --- Added Appendix southern bean mosaic virus related information. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. to Cui, Yang Cuiyun, Hupei Long, Tao Tingdian, India Liping. This standard replaces the standards previously issued as follows. --- SN/T 1611-2005. Southern bean mosaic virus serological methods

1 Scope

This standard specifies the plant quarantine in southern bean mosaic virus based on serological DAS-ELISA, immune electron microscopy and immuno-capture RT-PCR and other testing methods. This standard applies to detect the entry and exit legume seeds, seedlings and mass poisoning mediator in southern bean mosaic virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled SN/T 1840 plant virus immune electron microscopy method

3 Southern bean mosaic virus Basic Information

Chinese name. Southern bean mosaic virus Synonyms. bean mosaic virus No. 4 Beanmosaicvirus4; Southern bean mosaic virus 1 Southernbeanmosaicvirus1; dish Southern bean mosaic virus Beansouthernmosaicvirus. English name. Southernbeanmosaicvirus Abbreviation. SBMV Taxonomic position. Southern bean mosaic virus genus Sobemovirus Transmission. the virus may be spread by a round-robin type fashion leaf beetle. Bean seed transmission probability of 1% to 5%, cowpea seed-borne probability of 5% 40%. Mechanical inoculation experiments easy to spread. Other relevant information of the virus in Appendix A. Principle 4 SBMV spherical particles with a diameter of about 30nm for an effective immune source, can be prepared high titer antisera. Established on the basis of The DAS-ELISA and immune electron microscopy methods. Single split virus genome, according to the conserved sequence specific primers were designed, the establishment of a Free Phytophthora capture RT-PCR validation methods.

5 Equipment and Reagents

5.1 Equipment PCR instrument, the Reader, washer, electronic scales (sense of volume 1/1000g), electrophoresis, electrophoresis tank, gel imaging system, transmission electron microscopy Mirror, water bath, high-speed refrigerated centrifuge, small desktop centrifuge, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave, whirlpool Oscillator. 5.2 Reagents Unless otherwise stated, all test reagents were analytical grade. Southern bean mosaic virus serological test kits, RNA reverse ......

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