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SN/T 3576-2013 English PDF

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US$209.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 3576-2013: Detection of genetically modified components. Method for soybean using PCR-DHPLC
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3576-2013	209	Add to Cart	3 days	Detection of genetically modified components. Method for soybean using PCR-DHPLC	Valid

Similar standards

GB 1352 NY/T 1933 GB/T 10460

Basic data

Standard ID: SN/T 3576-2013 (SN/T3576-2013)
Description (Translated English): Detection of genetically modified components. Method for soybean using PCR-DHPLC
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B23
Classification of International Standard: 67.060
Word Count Estimation: 8,889
Quoted Standard: GB/T 19495.7; GB/T 6682; SN/T 1193
Regulation (derived from): AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the PCR-DHPLC detection of genetically modified soybean. This standard applies to genetically modified soybeans (roundup ready soybean, RRS) in the detection of genetically modified ingredients.

SN/T 3576-2013: Detection of genetically modified components. Method for soybean using PCR-DHPLC

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of genetically modified components. Method for soybean using PCR-DHPLC People's Republic of China Entry-Exit Inspection and Quarantine Standards GMO detection Soybean PCR-DHPLC Detection Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection Quarantine Science Research Institute, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., National Center for Nanoscience, Liaoning Academy of Agricultural Sciences. The main drafters. Zhang Guiming, water Zhu Fang, only to Yu, Cheng Yinghui, apricot Ling, Wang Zhaohui, PLAIN, Huang Xin, Ouyang Hui, Wang Ying, Zhong Jianzhong, Miao Jian Kun. GMO detection Soybean PCR-DHPLC Detection

1 Scope

This standard specifies the PCR-DHPLC detection of genetically modified soybean. This standard applies to genetically modified soybeans (roundupreadysoybean, RRS) in the detection of genetically modified ingredients.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.7 GMO detection methods of sampling and sample preparation SN/T 1193 genetic testing laboratory technical requirements Principle 3 Denaturing high-performance liquid chromatography (denaturinghigh-performanceliquidchromatography, DHPLC) is a simple Single, fast, non-gel method for nucleic acid analysis, samples were analyzed at 50 ℃ conditions, the number of base pairs in the sample determines the order of elution peak, when over Column acetonitrile concentration increases, the nucleic acid fragment will be from small to large order was eluted according to the relative molecular mass. This standard uses the multiplex PCR The method for genetically modified soybeans containing genetically modified simultaneously amplified PCR products were analyzed by DHPLC, obtained by DHPLC Elution peak marker to determine the relative molecular mass size comparison, it is determined whether or not having a heterologous gene composition and whether the genetically modified soybeans. 4 equipment, utensils and reagents 4.1 Equipment and Utensils Tissue grinder, PCR instrument, DHPLC meter, water bath, electronic balance (precision. 1/1000 above), ordinary centrifuge, freezing high Speed centrifuges, refrigerators, cold refrigerator, ice maker, oven temperature, pH meter, pipettes (0.1μL, 0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), UV-visible spectrophotometer, water machines, autoclaves, PCR tubes (0.2mL), centrifuge tubes (1.5mL, 2.0mL), Tip head (0.1μL ~ 10μL, 5μL ~ 200μL, 100μL ~ 500μL). 4.2 Reagents CTAB extract, Tris saturated phenol, chloroform, isoamyl alcohol, isopropyl alcohol, 70% ethanol, RNA enzyme (10mg/mL), multiplex PCR The reaction buffer solution (MultiplexPCRMix), TaqDNA polymerase, DHPLC Buffer A. TEAA (0.1mol/L, pH7.0), B Nitrile (0.025%), DHPLC Buffer B. TEAA (0.1mol/L, pH7.0), acetonitrile (25%), DHPLC buffer D. acetonitrile (75%, pH7.0), molecular weight marker (marker).

5 Sampling and sample preparation

According to GB/T 19495.7 performed. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 3576-2013_English be delivered?

Answer: Upon your order, we will start to translate SN/T 3576-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 3576-2013_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 3576-2013_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.