SN/T 1499-2004 English PDF

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SN/T 1499-2004: Determination of kojic acid in cosmetics. Liquid chromatography
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1499-2004	239	Add to Cart	3 days	Determination of kojic acid in cosmetics. Liquid chromatography	Obsolete

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Basic data

Standard ID: SN/T 1499-2004 (SN/T1499-2004)
Description (Translated English): Determination of kojic acid in cosmetics. Liquid chromatography
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: Y42
Classification of International Standard: 71.100.70
Word Count Estimation: 6,662
Date of Issue: 2004-11-17
Date of Implementation: 2005-04-01
Regulation (derived from): 2005 Bulletin No. 3 industry standard
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the kojic acid in cosmetics by HPLC detection method. This standard applies to kojic acid in cosmetics testing.

SN/T 1499-2004: Determination of kojic acid in cosmetics. Liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of kojic acid in cosmetics.Liquid chromatography Book of the People's Republic of China Entry and Exit Inspection and Quarantine Method for detecting kojic acid in cosmetics Liquid chromatography Released on November 11,.2004 2005-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the China Academy of Inspection and Quarantine. The main drafters of this standard. Liu Song, Lin Yuanhui, Chen Wei. This standard is the first industry standard for entry-exit inspection and quarantine. Method for detecting kojic acid in cosmetics Liquid chromatography

1 Scope

This standard specifies the liquid chromatography method for the determination of kojic acid in cosmetics. This standard applies to the detection of kojic acid in cosmetics.

2 Principle

The kojic acid in the cosmetics is extracted with water, filtered, and separated and measured by reversed-phase high performance liquid chromatography. According to its retention time Qualitative, standard work curve method quantitative.

3 reagents and materials

Unless otherwise stated, the reagents used were of analytical grade and the water was secondary deionized water. 3.1 Methanol. chromatographically pure. 3.2 Potassium dihydrogen phosphate. 0.02mol/L 3.3 kojic acid. purity ≥ 99%; 3.4 Quacic acid standard stock solution (200mg/L). Weigh 0.02g kojic acid, accurate to 0.1mg, in a 50mL beaker, add appropriate amount of water to dissolve, The solution was quantitatively transferred into a 100 mL volumetric flask, diluted to the mark with water, and mixed.

4 instruments

4.1 Liquid chromatograph with UV detector. 4.2 Microsampler, 10 μL. 4.3 Ultrasonic cleaner. 4.4 Centrifuge, 12000r/min. 4.5 Solvent filter and 0.45 μm filter membrane.

5 Determination steps

5.1 Sample processing Weigh about 0.5g of cosmetic sample, accurate to 1mg, in a 50mL plugged conical flask, add 20mL of water, in the ultrasonic cleaner The medium was shaken for 20 min, the solution was transferred to a 50 mL volumetric flask, diluted with water to the mark, and mixed. Take a part of the solution into the centrifuge tube, After centrifugation at 12000 r/min for 10 min in a centrifuge, the supernatant after centrifugation was filtered through a 0.45 μm filter, and the filtrate was used for measurement. 5.2 Determination 5.2.1 Chromatographic conditions 5.2.1.1 Column. ODSC18 column [250 mm × 4.6 mm (inside diameter), 5 μm, or equivalent]. 5.2.1.2 Mobile phase. methanol. 0.02 mol/L potassium dihydrogen phosphate = 18.82 (volume fraction). 5.2.1.3 Flow rate. 1.0mL/min; 5.2.1.4 Detection wavelength. 273 nm; 5.2.1.5 Column temperature. room temperature; 5.2.1.6 Injection volume. 10 μL. ......

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