SN/T 1394-2004 English PDFUS$199.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1394-2004: Protocol of milk ring test for brucellosis Status: Obsolete
Basic dataStandard ID: SN/T 1394-2004 (SN/T1394-2004)Description (Translated English): Protocol of milk ring test for brucellosis Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.22 Word Count Estimation: 4,483 Date of Issue: 6/1/2004 Date of Implementation: 12/1/2004 Regulation (derived from): State-Quality-Inspection-accreditation (2010) 582 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China Summary: This standard specifies the technical requirements for the operation of brucellosis whole milk ring test; This standard applies to such cattle brucellosis lactating cow disease monitoring and diagnosis of the disease brucellosis screening test does not apply to suffer from breast Mastitis and other breast diseases cow milk, colostrum, milk and boiled skim milk, does not apply to corruption, had soured and freezing; . SN/T 1394-2004: Protocol of milk ring test for brucellosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Protocol of milk ring test for brucellosis Book of the People's Republic of China Entry and Exit Inspection and Quarantine Brucellosis whole milk ring test method Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhu Shiqiang, Wang Yuling, Mi Baoguo. This standard was first published Entry and exit inspection and quarantine industry standards. Brucellosis whole milk ring test method1 ScopeThis standard specifies the operational technical requirements for the Brucellosis Whole Milk Ring Test. This standard applies to the monitoring and diagnosis of brucellosis in dairy cattle. The screening test for brucellosis in lactating cows is not applicable to breast cancer. Inflammation and other breast disease cows' milk, colostrum, skim milk and boiled milk are also not suitable for use in spoilage, sour and frozen milk.2 reagents and materials2.1 Reagents Standard antigen. tetrazolium staining antigen (red), or hematoxylin staining antigen (blue), stored in the dark at 2 °C ~ 8 °C. 2.2 Milk sample The test milk should be fresh whole milk. When collecting milk, wash the breasts of the dams with warm water, then dry them with clean gauze and squeeze the milk into cleanliness. In the utensils. If the milk sample is stored at 2 ° C ~ 8 ° C, it can be used within 7 days. 2.3 Materials 2.3.1 Micropipettes and tips. 2.3.2 Agglutination test tube. glass, the specification is 13mm × 100mm, round bottom, clean. 2.3.3 Test tube rack. 2.3.4 Water bath. 2.3.5 Pipette or graduated pipette.3 method of operation3.1 Take 1 mL of milk sample and add it to the sterilization agglutination test tube. 3.2 After fully shaking and mixing the standard antigen, remove 50 μL of the added milk sample and mix well. 3.3 Set in a 37 ° C ~ 38 ° C water bath for 60 min. 3.4 Take out the test tube, avoid oscillation, and make a judgment immediately.4 result determination4.1 Principle of judgment Determined by the color of the cream and the milk column. When the antigen was stained with tetrazolium, the color was red, and the color of the antigen stained with hematoxylin was blue. When the milk with less fat or no fat is positive, the cells are agglomerated and sink the bottom of the tube. The judgment is based on the reaction of the milk column. 4.2 Judging criteria 4.2.1 Positive reaction (+++). The upper layer of milk in the milk column forms a red or blue ring with a clear white color and a clear definition. 4.2.2 Positive reaction (++). The annulus of the cream layer is red or blue, but not significant, and the breast is slightly colored. 4.2.3 Weak positive reaction (+). The ring of the cream layer is lighter in color but slightly darker than the color of the milk column. 4.2.4 Suspected reaction (±). The color of the ring of the cream layer is not obvious, the boundary with the milk column is unclear, and the milk column does not fade. 4.2.5 Negative reaction (-). There is no change in the upper layer of the milk column, and the color of the milk column is uniform. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1394-2004_English be delivered?Answer: Upon your order, we will start to translate SN/T 1394-2004_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 2 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1394-2004_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1394-2004_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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