SN/T 1396-2015 English PDF

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SN/T 1396-2015: Quarantine protocol for toxoplasmosis
Status: Valid

SN/T 1396: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1396-2015	349	Add to Cart	3 days	Quarantine protocol for toxoplasmosis	Valid
SN/T 1396-2004	239	Add to Cart	2 days	Toxoplasmosis indirect hemagglutination test	Obsolete

Similar standards

GB/T 20365   SN/T 1353   NY/T 909   SN/T 5663   SN/T 5671   SN/T 1395   

Basic data

Standard ID: SN/T 1396-2015 (SN/T1396-2015)
Description (Translated English): Quarantine protocol for toxoplasmosis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 15,151
Date of Issue: 2015-09-02
Date of Implementation: 2016-04-01
Older Standard (superseded by this standard): SN/T 1396-2004
Regulation (derived from): AQSIQ Announcement 2015 - Batch 3 - 103 Industry Standards
Issuing agency(ies): General Administration of Customs

SN/T 1396-2004: Toxoplasmosis indirect hemagglutination test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Toxoplasmosis indirect hemagglutination test Book of the People's Republic of China Entry and Exit Inspection and Quarantine Indirect hemagglutination test for toxoplasmosis Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Hubei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Chen Jianjun, Guo Mingxing, Fu Wenpeng, Zhao Hui and so on. This standard is the first industry standard for entry-exit inspection and quarantine. Indirect hemagglutination test for toxoplasmosis

1 Scope

This standard specifies the indirect hemagglutination (IHA) test method for toxoplasmosis. This standard applies to the quarantine, diagnosis and detection of antibody levels of toxoplasmosis in animals.

2 Materials and reagents

2.1 96 (12 × 8) hole 110 ° V type blood coagulation plate and glass cover. 2.2 Adjustable micropipettes and tips. 2.3 Plate oscillator. 2.4 Toxoplasma IHA diagnostic solution (antigen titer ≥ 1.1024), standard negative control serum (potency ≤ 1.4), standard positive control serum (potency ≥ 1. 1024). 2.5 The dilutions were prepared as indicated in Appendix A. The reagents used were of analytical grade.

3 sampling

The venous blood of the aseptic animal is about 1 mL to 2 mL, and the serum is naturally precipitated or the blood is coagulated, and the serum is centrifuged at 600 g. Inactivated in a 56 ° C water bath for 30 min, pre-cooled, and incubated with healthy ram red blood cells (mixed at a serum/erythrocyte volume ratio of about 1.1) for 2 h at room temperature. (or 4 ° C refrigerator overnight), centrifuged at 600g for 10min, take the supernatant (requires no blood cells, no hemolysis, no contamination of bacteria), set the refrigerator at 4 °C for inspection.

4 steps

4.1 Take a clean hemagglutination plate and add 75 μL of the dilution to each well. 4.2 Take 25 μL of the test serum into the first column of wells, pipette and mix repeatedly, take 25 μL into the second column of wells, mix and take Add 25 μL to the third column of wells, so dilute to the 6th well 4 times, mix and dispose 25 μL at 1.4096 (diluted only for large samples) See 1.1 to 1.64. Table 1 Indirect hemagglutination test operation for toxoplasmosis Hole number 1 2 3 4 5 6 7 Serum dilution (1. ) 4 16 64 256 1024 4096 Diluent empty White control Diluent/μL Tested serum/μL 75}25 75}25 75}25 75}25 75}25 75}25 75 abandon 25 Diagnostic solution/μL 25 25 25 25 25 25 25 Feeling 22 ° C ~ 37 ° C for 2h ~ 3h Example of results (like) ++++ ++++ ++ - - - - 4.3 Each test shall be used as a standard negative, positive serum and diluent blank control. The standard positive serum control should be diluted to 1.4096. The quasi-negative serum control should be diluted to 1.64, and the standard negative and positive serum dilution methods are the same as the test serum. 4.4 Take the Toxoplasma IHA diagnostic solution (if it is a lyophilized product, use it after washing and dilution according to the instructions for use), add 25 μL to each test well. Shake the oscillator for about 1 minute (or gently hold the hemagglutination plate around the circle until the blood cells are evenly distributed in each hole), cover the glass cover, 22 ° C ~ 37 ° C ......

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