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SN/T 1314-2003 English PDF

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US$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1314-2003: Protocol of counter immunoelectrophoresis for mink aleutian disease
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1314-2003	239	Add to Cart	3 days	Protocol of counter immunoelectrophoresis for mink aleutian disease	Obsolete

Similar standards

GB/T 20365 SN/T 1353 NY/T 909 SN/T 5671 SN/T 1316 SN/T 1315

Basic data

Standard ID: SN/T 1314-2003 (SN/T1314-2003)
Description (Translated English): Protocol of counter immunoelectrophoresis for mink aleutian disease
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 6,687
Date of Issue: 2003-08-18
Date of Implementation: 2004-02-01
Quoted Standard: GB/T 6682
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the Aleutian mink disease counter immuno electrophoresis assay procedures. This standard applies to a counter immuno electrophoresis to detect enter, exit Aleutian mink disease antibodies.

SN/T 1314-2003: Protocol of counter immunoelectrophoresis for mink aleutian disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of counter immunoelectrophoresis for mink aleutian disease Book of the People's Republic of China Entry and Exit Inspection and Quarantine Oyster Aleutian disease convective immunoelectrophoresis operation procedure Released on.2003-08-18 2004-02-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard electrophoresis operation method refers to the quarantine method of the Animal Quarantine Operation Regulations (1988 edition) of the Ministry of Agriculture, and has developed sample loading. Pre-processing method. Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Lai Pingan, Zhang Hexiao, Liu Yanhua. This standard is the industry standard for inspection and quarantine issued for the first time. Oyster Aleutian disease convective immunoelectrophoresis operation procedure

1 Scope

This standard specifies the operating procedures for convective immunoelectrophoresis testing of leech Aleutian disease. This standard is applicable to the detection of influent Aleutian disease antibodies by convection immunoelectrophoresis.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods

3 Material preparation

3.1 Instruments and equipment 3mm thick 100mm × 45mm glass plate; voltage 0V ~ 300V, current 0mA ~ 50mA electrophoresis instrument; width 100mm Electrophoresis tank; 3mm diameter puncher; 100μL micro-adjustable pipette and matching tip; plastic capillary blood collection tube. Barbital sodium 10.31g Tris-hydroxymethylaminomethane 6.005g Sodium azide 0.2g Secondary water (in accordance with GB/T 6682) 1000mL After adding the appropriate amount of secondary water (according to GB/T 6682), dissolve the pH value to 8.5-8.6 with hydrochloric acid, and finally use the second grade. Water (in accordance with GB/T 6682) to a volume of 1000mL. 3.3 agarose Analytically pure agarose for domestic or imported electrophoresis. 3.4 antigen It was prepared from a cat kidney passage cell culture of the Aleutian virus international standard strain Utah-1. Provided by the designated unit. 3.5 serum 3.5.1 Positive serum. provided by the designated unit. 3.5.2 Negative serum. provided by the designated unit. 3.5.3 Serum to be tested. Blood is collected from the tip of the toe with a plastic capillary tube. The two ends of the tube are sealed and sealed, placed in an environment of 20 ° C to 30 ° C for 1 h, and then placed. 4 ° C refrigerator 18h ~ 20h, precipitated serum, add 0.5mL or 1.5mL centrifuge tube, centrifuge at 3000r/min for 5min, take the supernatant spare.

4 operating procedures

4.1 Boarding Take 3mm thick 100mm × 45mm horizontal scratch-free glass plate, wash the oil, put it on the horizontal table, use 10mL The pipette draws 0.8% agarose buffer which has been dissolved and cooled to about 70 ° C, and is evenly spread on a glass plate with a thickness of 3 mm. When the glass plate is surrounded by a baffle, it will be used after cooling and solidification, and it will be used up on the same day. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1314-2003_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1314-2003_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1314-2003_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1314-2003_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.