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SN/T 1316-2011 English PDF

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SN/T 1316-2011: Quarantine protocol for bovine spongiform encephalopathy
Status: Valid

SN/T 1316: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1316-2011769 Add to Cart 6 days Quarantine protocol for bovine spongiform encephalopathy Valid
SN/T 1316-2003319 Add to Cart 3 days Protocol of histopathological diagnosis for bovine spongiform encephalopathy Obsolete

Similar standards

GB/T 20365   SN/T 1353   NY/T 909   SN/T 5663   SN/T 5671   SN/T 1315   

Basic data

Standard ID: SN/T 1316-2011 (SN/T1316-2011)
Description (Translated English): Quarantine protocol for bovine spongiform encephalopathy
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 31,312
Date of Issue: 2011-05-31
Date of Implementation: 2011-12-01
Older Standard (superseded by this standard): SN/T 1316-2003
Quoted Standard: GB/T 6682; GB 19489
Regulation (derived from): National-Quality-Inspection-accreditation (2011) 291
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the sample of bovine spongiform encephalopathy, histopathological examination methods, immunohistochemistry, protein immunoblotting, ELISA test methods, technical requirements immunochromatographic strip detection methods, such as detection methods and operations specification. Histopathological examination method in which an auxiliary method of immunohistochemistry. SAF immunohistochemistry and Western blot diagnostic method for the diagnosis. Western blot method, ELISA method and immunochromatographic test strip detection method for screening methods. This standard applies to the diagnosis of bovine spongiform encephalopathy, can also be used for the diagnosis of transmissible spongiform encephalopathies other animals.

SN/T 1316-2011: Quarantine protocol for bovine spongiform encephalopathy

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for bovine spongiform encephalopathy People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1316-2003 BSE Technical Specifications quarantine Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1316-2003 "bovine spongiform encephalopathy histopathological examination methods." This standard compared with SN/T 1316-2003, major technical changes are as follows. --- Increased BSE sampling; --- Increasing the Western blot method; --- Increased enzyme linked immunosorbent assay method (ELISA); --- Increased immunochromatographic strip for detection methods. The standard reference of the International Organization for Animal Health (OIE) of "diagnostic tests and vaccines Standards Manual" (2008 Edition) "ManualofDiag- nosticTestsandVaccinesforTerrestrialAnimals ") in Chapter 2.4.6" BSE diagnostic techniques. " This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Beijing Exit Inspection and Quarantine drafted. The main drafters of this standard. Ma Guiping, Liyan Xin, Li Bingling, Liu Quanguo, Shixi Ju, Liu Xuhui. This standard replaces the standards previously issued as follows. --- SN/T 1316-2003. BSE Technical Specifications quarantine

1 Scope

This standard specifies the sampling of bovine spongiform encephalopathy histopathological examination method, immunohistochemistry, Western blot method, Technical requirements ELISA test methods, immunochromatographic strip detection methods and other detection methods and practices. Wherein Histopathology Examination method immunohistochemistry helper methods, immunohistochemistry and Western blot SAF diagnosed as confirmed side Method, Western blot method, ELISA method and immunochromatographic strip for detection method for the screening method. This standard applies to the diagnosis of bovine spongiform encephalopathy, also be used for the diagnosis of transmissible spongiform encephalopathies other animals.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety

3 clinical diagnosis

3.1 Bovine Spongiform Encephalopathy (BSE) average incubation period of 4 years to 5 years. Cows clinical manifestations of mental disorders, movement disorders and sensory obstacle. 3.2 psychiatric disorders. mainly as anxiety, fear, rage, etc., or when someone close to persecute them when often appear aggressive behavior. 3.3 movement disorders. mainly as ataxia, tremor or fall. Cow gait was "goose step" shaped limbs hyperextension, hind limb motor loss Tone, tremors and falls, paralysis, paresis; sometimes fell hard to stand. 3.4 sensory dysfunction. The most common is a touch over-sensitive sound and light, this is a very important clinical BSE cow diagnostic features. Hand Touch or touch and pressure with a blunt neck rib cattle, cows will be extremely nervous trembling, light touch with a broom heels, there will be tension kicks reaction; disease Cattle knocker metal instrument sounds, there will be shocked and trembling reaction; cows in a dark environment, the sudden opening of the lights appeared frightened And shivering reaction.

4 Laboratory diagnosis

4.1 Sampling and sample preparation methods 4.1.1 Materials 4.1.1.1 subject tissue Be seized bovine brain. 4.1.1.2 Reagents 10% neutral formalin solution (see Appendix A); disinfectant. 2% sodium hypochlorite, 2mol/L sodium hydroxide; 98% to 99% formic acid; no Aqueous ethanol; 95% ethanol; 70% ethanol solution; xylene; paraffin wax (melting point 54 ℃ ~ 56 ℃, 56 ℃ ~ 58 ℃, 58 ℃ ~ 60 ℃).
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