SN/T 1182-2010 English PDF

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SN/T 1182-2010: Quarantine protocol for avian influenza
Status: Valid

SN/T 1182: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1182-2010	709	Add to Cart	5 days	Quarantine protocol for avian influenza	Valid
SN/T 1182.1-2003	239	Add to Cart	2 days	Detection antibodies against avian influenza virus. Protocol of agar gel immuno-diffusion test	Obsolete

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Basic data

Standard ID: SN/T 1182-2010 (SN/T1182-2010)
Description (Translated English): Quarantine protocol for avian influenza
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 27,241
Date of Issue: 2010-11-01
Date of Implementation: 2011-05-01
Older Standard (superseded by this standard): SN/T 1182.1-2003; SN/T 1182.2-2004
Quoted Standard: GB/T 19438.1; GB/T 19438.2; GB/T 19438.3; GB/T 19438.4; GB 19489
Adopted Standard: WHO/CDS/CSR/NCS/2002.5 Rev.1, NEQ
Regulation (derived from): National Quality Inspection (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of poultry and its products avian flu quarantine specifications. This standard applies to bird flu quarantine.

SN/T 1182.1-2003: Detection antibodies against avian influenza virus. Protocol of agar gel immuno-diffusion test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection antibodies against avian influenza virus.Protocol of agar gel immuno-diffusion test Book of the People's Republic of China Entry and Exit Inspection and Quarantine Avian influenza antibody detection method Agar immunodiffusion test Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is based on OIE/Chapter 2.1.14, 4thedition,.2000, Manual on Diagnostic Methods and Vaccine Standards (Manualofstand- Ardsfordiagnostictestsandvaccines) drafted. Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Pan Wenbo, Xue Jingshan, Xu Haini, Liao Xiuyun. This standard is the industry standard for inspection and quarantine issued for the first time. Avian influenza antibody detection method agar immunodiffusion test

1 Scope

This standard specifies the operating procedures for avian influenza agar immunodiffusion test. This standard applies to the detection of avian influenza (AI) virus antibodies in avian serum. Mainly applicable to quarantine and epidemic situation of poultry avian influenza Monitoring and epidemiological investigations.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods

3 Principle

The molecular weight of antigens and antibodies is generally below.200,000 Daltons, and antigens and their specific antibodies are low in the gel from high concentration areas. When the concentration region diffuses, the resistance is small and is basically in the form of free diffusion. Due to the molecular weight, structure, shape and electricity of different antigen molecules The charge is different, so the diffusion coefficient is different and the diffusion speed in the gel is different. When the antigen and the corresponding antibody are diffused in the gel When they meet, an antigen-antibody complex is formed, and if the ratio of the two is appropriate at the meeting, the largest complex is formed. Due to the increased molecular weight of the complex Large, the particles increase, and thus no longer continue to diffuse to produce a precipitate, appearing in the form of a line or a band, and the formation of a line or band is called immunoprecipitation. Line or immunoprecipitation zone, therefore, whether the presence of specificity in the serum to be tested can be determined based on whether a sedimentation line appears between the antigen and the test serum. antibody.

4 Material preparation

4.1 Reagents. sodium dihydrogen phosphate (NaH2PO4·2H2O), disodium hydrogen phosphate (Na2HPO4·12H2O), sodium chloride (NaCl), azide Sodium, agarose, were of analytical grade. 4.2 Agar diffusion test antigen, standard positive serum. supplied by the designated unit. Use according to the instructions. 4.3 Equipment. straw, measuring cylinder, plate (size. 90mm × 15mm), triangular bottle, metal punch with outer diameter of about 5mm, micro pipette And micro tips, observation lights, etc. 4.4 Serum to be tested. Serum is collected by routine blood collection. There should be no hemolysis and no bacterial contamination. Store at 4 °C.

5 Method of operation

5.1 Test water The water used in this standard should meet the specifications of the third grade water (three steamed water) in GB/T 6682-1992. 5.2 Preparation of agar plates 5.2.1 See Appendix A for the preparation of the agar solution. 5.2.2 Plate. When the agar solution is naturally cooled to about 56 ° C, a 90 mm diameter clean and sterilized plate is placed on a horizontal platform, each Add 15mL to the plate, make the gel thickness 2mm ~ 3mm, cover the plate cover, after the natural cooling and solidification, put the plate into the wet box, 4 ° C ......

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