SN/T 1181-2010 English PDF

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SN/T 1181-2010: Quarantine protocol for foot and mouth disease
Status: Valid

SN/T 1181: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1181-2010	599	Add to Cart	4 days	Quarantine protocol for foot and mouth disease	Valid
SN/T 1181.1-2003	279	Add to Cart	3 days	Detection antibodies against foot and mouth disease virus infection-associated antigen. Protocol agar gel immuno-diffusion test	Obsolete

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Basic data

Standard ID: SN/T 1181-2010 (SN/T1181-2010)
Description (Translated English): Quarantine protocol for foot and mouth disease
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 23,273
Date of Issue: 2010-11-01
Date of Implementation: 2011-05-01
Older Standard (superseded by this standard): SN/T 1181.1-2003; SN/T 1181.2-2003; SN/T 1181.3-2003
Quoted Standard: GB/T 6682
Regulation (derived from): National Quality Inspection (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the FMD virus isolation and identification, enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, fluorescence reverse transcription polymerase chain reaction, virus neutralization test, the solid phase competitive enzyme-linked immunosorbent assay, liquid phase blocking ELISA, and agar immunodiffusion test specifications. This standard applies to FMD quarantine.

SN/T 1181.1-2003: Detection antibodies against foot and mouth disease virus infection-associated antigen. Protocol agar gel immuno-diffusion test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection antibodies against foot and mouth disease virus infection-associated antigen.Protocol agar gel immuno-diffusion test Book of the People's Republic of China Entry and Exit Inspection and Quarantine Foot and mouth disease virus infection antibody detection method Agar immunodiffusion test Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is based on OIE/Chapter 2.1.1, 4thedition,.2000, Diagnostic Test and Vaccine Standards Manual (Manualofstand- ardsfordiagnosticTestsandVaccines) drafted. Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Xu Haini, Xue Jingshan, Pan Wenbo, Liao Xiuyun. This standard is the industry standard for inspection and quarantine issued for the first time. Foot and mouth disease virus infection antibody detection method Agar immunodiffusion test

1 Scope

This standard specifies the operating procedures for agar immunodiffusion test of foot-and-mouth disease virus infection-related antigens. This standard is applicable to the detection of antibodies against foot-and-mouth disease virus-associated antigen (VIA) in susceptible animal serum, and is applicable to all types of foot-and-mouth disease. Quarantine (no type specificity), epidemic surveillance and epidemiological investigation.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this standard. 3.1 Agar immunodiffusion test. 3.2 Foot and mouth disease. 3.3 The virus infects the relevant antigen. 3.4 Trishydroxymethylaminomethane-hydrochloric acid solution.

4 Principle

The antigen and its specific antibody have little resistance when diffused from the high concentration region to the low concentration region in the gel, and are basically freely expanded. Form of dispersion. Since different molecular molecules differ in molecular weight, structure, shape, and charge amount, their diffusion coefficients are different. When antigen and corresponding After the antibodies are diffused, they meet in the gel to form an antigen-antibody complex. If the ratio of the two is appropriate at the meeting, the largest complex is formed. As the molecular weight of the composite increases, the particles increase, and thus do not continue to diffuse to form a precipitate, which exhibits a linear or band shape, and forms a linear shape. Or a band-like "specific barrier" is called an immunoprecipitation line or an immunoprecipitation zone. Agar immunodiffusion test based on standard antigen and test serum Whether a sedimentation line appears between them determines whether a specific antibody is present in the serum to be tested. ......

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